NDLI: Parvovirus particles and movement in the cellular cytoplasm and effects of the cytoskeleton

Content Provider	PubMed Central
Author	Michael, Lyi Sangbom Tan, Min Jie Alvin Parrish, Colin R.
Copyright Year	2014
Abstract	Cell infection by parvoviruses requires that capsids be delivered from outside the cell to the cytoplasm, followed by genome trafficking to the nucleus. Here we microinject capsids into cells that lack receptors and followed their movements within the cell over time. In general the capsids remained close to the positions where they were injected, and most particles did not move to the vicinity of or enter the nucleus. When 70 kDa-dextran was injected along with the capsids that did not enter the nucleus in significant amounts. Capsids conjugated to peptides containing the SV40 large T antigen nuclear localization signal remained in the cytoplasm, although bovine serum albumen conjugated to the same peptide entered the nucleus rapidly. No effects of disruption of microfilaments, intermediate filaments, or microtubules on the distribution of the capsids were observed. These results suggest that movement of intact capsids within cells is primarily associated with passive processes.
Related Links	http://dx.doi.org/10.1016/j.virol.2014.04.003
Ending Page	352
Page Count	11
Starting Page	342
File Format	PDF
ISSN	00426822
e-ISSN	10960341
Journal	Virology
Volume Number	0
Language	English
Publisher Date	2014-05-01
Access Restriction	Open
Subject Keyword	Virology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology

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Parvovirus particles and movement in the cellular cytoplasm and effects of the cytoskeleton