NDLI: The effect of local bending on gating of MscL using a representative volume element and finite element simulation

Content Provider	PubMed Central
Author	Bavi, Omid Vossoughi, Manouchehr Reza, Naghdabadi Jamali, Yousef
Copyright Year	2014
Abstract	Many physiological processes such as cell division, endocytosis and exocytosis cause severe local curvature of the cell membrane. Local curvature has been shown experimentally to modulate numerous mechanosensitive (MS) ion channels. In order to quantify the effects of local curvature we introduced a coarse grain representative volume element for the bacterial mechanosensitive ion channel of large conductance (MscL) using continuum elasticity. Our model is designed to be consistent with the channel conformation in the closed and open states to capture its major continuum rheological behavior in response to the local membrane curvature. Herein we show that change in the local curvature of the lipid bilayer can modulate MscL activity considerably by changing both bilayer thickness and lateral pressure profile. Intriguingly, although bending in any direction results in almost the same free-energy cost, inward (cytoplasmic) bending favors channel opening, whereas outward (periplasmic) bending facilitates closing of the narrowest part of the MscL pore. This quantitative study using MscL as a model channel may have wide reaching consequences for the effect of local curvature on the physiological function of other types of prokaryotic and eukaryotic membrane proteins.
Related Links	http://dx.doi.org/10.4161/chan.29572
Ending Page	349
Page Count	6
Starting Page	344
File Format	PDF
ISSN	19336950
e-ISSN	19336969
Journal	Channels
Issue Number	4
Volume Number	8
Language	English
Publisher	Landes Bioscience
Publisher Date	2014-05-01
Access Restriction	Open
Rights Holder	Landes Bioscience
Subject Keyword	Biophysics Biochemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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A Finite Element Framework for Studying the Mechanical Response of Macromolecules: Application to the Gating of the Mechanosensitive Channel MscL

Simulation of MscL Gating in a Bilayer under Stress

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