NDLI: Differentiation of rhesus adipose stem cells into dopaminergic neurons☆

Content Provider	PubMed Central
Author	Zhou, Yan Sun, Maosheng Li, Hongjun Yan, Min Xie, Tianhong
Copyright Year	2012
Abstract	LIM homeobox transcription factor 1a (Lmx1a) has the capacity to initiate the development program of neuronal cells and promote the differentiation of embryonic stem cells into dopaminergic neurons. In this study, rhesus adipose stem cells were infected with recombinant adenovirus carrying the Lmx1a gene and co-cultured with embryonic rat neural stem cells. Cell differentiation was induced using sonic hedgehog and fibroblast growth factor-8. Immunofluorescence staining showed that cells were positive for neuron-specific enolase and β-tubulin III. Reverse transcription-PCR results demonstrated that rhesus adipose stem cells were not only positive for neuron-specific enolase and β-tubulin III, but also positive for the dopaminergic neuron marker, tyrosine hydroxylase, neurofilament, glial cell line-derived neurotrophic factor family receptor α2 and nuclear receptor related factor 1. The number of Lmx1a gene-infected cells expressing the dopaminergic neuron marker was substantially greater than the number of cells not infected with Lmx1α gene. These results suggest that Lmx1a-mediated regulation combined with the strategy of co-culture with neural stem cells can robustly promote the differentiation of rhesus adipose stem cells into dopaminergic neurons.
Related Links	http://dx.doi.org/10.3969/j.issn.1673-5374.2012.34.001
Starting Page	2645
File Format	PDF
ISSN	18767958
e-ISSN	18767958
Journal	Neural Regeneration Research
Issue Number	34
Volume Number	7
Language	English
Publisher	Medknow Publications & Media Pvt Ltd
Publisher Date	2012-12-05
Access Restriction	Open
Rights Holder	Medknow Publications & Media Pvt Ltd
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Developmental Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons★

Genetic engineering of mouse embryonic stem cells by Nurr1 enhances differentiation and maturation into dopaminergic neurons

Differentiation of human adipose-derived stem cells into neuron-like cells by Radix Angelicae Sinensis

Proneural Transcription Factor Atoh1 Drives Highly Efficient Differentiation of Human Pluripotent Stem Cells Into Dopaminergic Neurons

Differentiation of Wharton's jelly mesenchymal stem cells into neurons in alginate scaffold

Growth Factors and Feeder Cells Promote Differentiation of Human Embryonic Stem Cells into Dopaminergic Neurons: A Novel Role for Fibroblast Growth Factor-20

Differentiation of Human Epidermal Neural Crest Stem Cells (hEPI-NCSC) into Virtually Homogenous Populations of Dopaminergic Neurons

Expression of Early Developmental Markers Predicts the Efficiency of Embryonic Stem Cell Differentiation into Midbrain Dopaminergic Neurons

Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes

Differentiation of rhesus adipose stem cells into dopaminergic neurons☆

Similar Documents

Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons★

Genetic engineering of mouse embryonic stem cells by Nurr1 enhances differentiation and maturation into dopaminergic neurons

Differentiation of human adipose-derived stem cells into neuron-like cells by Radix Angelicae Sinensis

Proneural Transcription Factor Atoh1 Drives Highly Efficient Differentiation of Human Pluripotent Stem Cells Into Dopaminergic Neurons

Differentiation of Wharton's jelly mesenchymal stem cells into neurons in alginate scaffold

Growth Factors and Feeder Cells Promote Differentiation of Human Embryonic Stem Cells into Dopaminergic Neurons: A Novel Role for Fibroblast Growth Factor-20

Differentiation of Human Epidermal Neural Crest Stem Cells (hEPI-NCSC) into Virtually Homogenous Populations of Dopaminergic Neurons

Expression of Early Developmental Markers Predicts the Efficiency of Embryonic Stem Cell Differentiation into Midbrain Dopaminergic Neurons

Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes

Differentiation of rhesus adipose stem cells into dopaminergic neurons☆