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| Content Provider | PubMed Central |
|---|---|
| Author | Nogueira, Cristina Helena, Kashevsky Pinto, Belinda Clarke, Astrid Orr-weaver, Terry L. |
| Copyright Year | 2014 |
| Abstract | The Shugoshin (Sgo) protein family helps to ensure proper chromosome segregation by protecting cohesion at the centromere by preventing cleavage of the cohesin complex. Some Sgo proteins also influence other aspects of kinetochore-microtubule attachments. Although many Sgo members require Aurora B kinase to localize to the centromere, factors controlling delocalization are poorly understood and diverse. Moreover, it is not clear how Sgo function is inactivated and whether this is distinct from delocalization. We investigated these questions in Drosophila melanogaster, an organism with superb chromosome cytology to monitor Sgo localization and quantitative assays to test its function in sister-chromatid segregation in meiosis. Previous research showed that in mitosis in cell culture, phosphorylation of the Drosophila Sgo, MEI-S332, by Aurora B promotes centromere localization, whereas Polo phosphorylation promotes delocalization. These studies also suggested that MEI-S332 can be inactivated independently of delocalization, a conclusion supported here by localization and function studies in meiosis. Phosphoresistant and phosphomimetic mutants for the Aurora B and Polo phosphorylation sites were examined for effects on MEI-S332 localization and chromosome segregation in meiosis. Strikingly, MEI-S332 with a phosphomimetic mutation in the Aurora B phosphorylation site prematurely dissociates from the centromeres in meiosis I. Despite the absence of MEI-S332 on meiosis II centromeres in male meiosis, sister chromatids segregate normally, demonstrating that detectable levels of this Sgo are not essential for chromosome congression, kinetochore biorientation, or spindle assembly. |
| Related Links | http://dx.doi.org/10.1534/g3.114.012823 |
| Ending Page | 1858 |
| Page Count | 10 |
| Starting Page | 1849 |
| File Format | |
| ISSN | 21601836 |
| e-ISSN | 21601836 |
| Journal | G3: Genes|Genomes|Genetics |
| Issue Number | 10 |
| Volume Number | 4 |
| Language | English |
| Publisher | Genetics Society of America |
| Publisher Date | 2014-07-31 |
| Access Restriction | Open |
| Rights Holder | Genetics Society of America |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Molecular Biology Genetics (clinical) |
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