NDLI: The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1

Content Provider	PubMed Central
Author	Ngo, Greg H. P. Balakrishnan, Lata Dubarry, Marion Campbell, Judith L. David, Lydall
Copyright Year	2014
Abstract	Single-stranded DNA (ssDNA) at DNA ends is an important regulator of the DNA damage response. Resection, the generation of ssDNA, affects DNA damage checkpoint activation, DNA repair pathway choice, ssDNA-associated mutation and replication fork stability. In eukaryotes, extensive DNA resection requires the nuclease Exo1 and nuclease/helicase pair: Dna2 and Sgs1BLM. How Exo1 and Dna2-Sgs1BLM coordinate during resection remains poorly understood. The DNA damage checkpoint clamp (the 9-1-1 complex) has been reported to play an important role in stimulating resection but the exact mechanism remains unclear. Here we show that the human 9-1-1 complex enhances the cleavage of DNA by both DNA2 and EXO1 in vitro, showing that the resection-stimulatory role of the 9-1-1 complex is direct. We also show that in Saccharomyces cerevisiae, the 9-1-1 complex promotes both Dna2-Sgs1 and Exo1-dependent resection in response to uncapped telomeres. Our results suggest that the 9-1-1 complex facilitates resection by recruiting both Dna2-Sgs1 and Exo1 to sites of resection. This activity of the 9-1-1 complex in supporting resection is strongly inhibited by the checkpoint adaptor Rad953BP1. Our results provide important mechanistic insights into how DNA resection is regulated by checkpoint proteins and have implications for genome stability in eukaryotes.
Related Links	http://dx.doi.org/10.1093/nar/gku746
Ending Page	10528
Page Count	13
Starting Page	10516
File Format	PDF
ISSN	03051048
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	16
Volume Number	42
Language	English
Publisher	Oxford University Press
Publisher Date	2014-09-15
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1

The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1

A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1–Dna2 in long-range DNA end resection

The 9-1-1 checkpoint clamp coordinates resection at DNA double strand breaks

DNA resection proteins Sgs1 and Exo1 are required for G1 checkpoint activation in budding yeast

The 9-1-1 checkpoint clamp coordinates resection at DNA double strand breaks

Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double strand break ends

Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2

DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2

The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1

Similar Documents

9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1

The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1

A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1–Dna2 in long-range DNA end resection

The 9-1-1 checkpoint clamp coordinates resection at DNA double strand breaks

DNA resection proteins Sgs1 and Exo1 are required for G1 checkpoint activation in budding yeast

The 9-1-1 checkpoint clamp coordinates resection at DNA double strand breaks

Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double strand break ends

Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2

DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2

The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1