NDLI: In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation

Content Provider	PubMed Central
Author	Gierut, Jessica J. Jacks, Tyler E. Haigis, Kevin M.
Copyright Year	2014
Abstract	Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation.
Related Links	http://dx.doi.org/10.1101/pdb.prot073445
Ending Page	309
Page Count	3
Starting Page	307
File Format	PDF
ISSN	15596095
e-ISSN	15596095
Journal	Cold Spring Harbor protocols
Issue Number	3
Volume Number	2014
Language	English
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation