NDLI: How does ethanol induce apoptotic cell death of SK-N-SH neuroblastoma cells

Content Provider	PubMed Central
Author	Moon, Yong Kwon, Yongil Yu, Shun
Copyright Year	2013
Abstract	A body of evidence suggests that ethanol can lead to damage of neuronal cells. However, the mechanism underlying the ethanol-induced damage of neuronal cells remains unclear. The role of mitogen-activated protein kinases in ethanol-induced damage was investigated in SK-N-SH neuroblastoma cells. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay, DNA fragmentation detection, and flow cytometric analysis showed that ethanol induced apoptotic cell death and cell cycle arrest, characterized by increased caspase-3 activity, DNA fragmentation, nuclear disruption, and G1 arrest of cell cycle of the SK-N-SH neuroblastoma cells. In addition, western blot analysis indicated that ethanol induced a lasting increase in c-Jun N-terminal protein kinase activity and a transient increase in p38 kinase activity of the neuroblastoma cells. c-Jun N-terminal protein kinase or p38 kinase inhibitors significantly reduced the ethanol-induced cell death. Ethanol also increased p53 phosphorylation, followed by an increase in p21 tumor suppressor protein and a decrease in phospho-Rb (retinoblastoma) protein, leading to alterations in the expressions and activity of cyclin dependent protein kinases. Our results suggest that ethanol mediates apoptosis of SK-N-SH neuroblastoma cells by activating p53-related cell cycle arrest possibly through activation of the c-Jun N-terminal protein kinase-related cell death pathway.
Related Links	http://dx.doi.org/10.3969/j.issn.1673-5374.2013.20.004
Starting Page	1853
File Format	PDF
ISSN	18767958
e-ISSN	18767958
Journal	Neural Regeneration Research
Issue Number	20
Volume Number	8
Language	English
Publisher	Medknow Publications & Media Pvt Ltd
Publisher Date	2013-07-15
Access Restriction	Open
Rights Holder	Medknow Publications & Media Pvt Ltd
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Developmental Neuroscience

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