NDLI: Body-on-a-Chip Simulation with Gastrointestinal Tract and Liver Tissues Suggests that Ingested Nanoparticles Have the Potential to Cause Liver Injury

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Body-on-a-Chip Simulation with Gastrointestinal Tract and Liver Tissues Suggests that Ingested Nanoparticles Have the Potential to Cause Liver Injury

Content Provider	PubMed Central
Author	Esch, Mandy B. Mahler, Gretchen J. Tracy, Stokol Shuler, Michael L.
Abstract	The use of nanoparticles in medical applications is highly anticipated, and at the same time little is known about how these nanoparticles affect human tissues. Here we have simulated the oral uptake of 50 nm carboxylated polystyrene nanoparticles with a microscale, body-on-a-chip system (also referred to as multi-tissue microphysiological system or micro Cell Culture Analog). Using this system, we combined in vitro models of the human intestinal epithelium, represented by a co-culture of enterocytes (Caco-2) and mucin-producing (HT29-MTX) cells, and the liver, represented by HepG2/C3A cells, within one microfluidic device. The device also contained chambers that together represented all other organs of the human body. Measuring the transport of 50 nm carboxylated polystyrene nanoparticles across the Caco-2/HT29-MTX co-culture, we have found that this multi-cell layer presents an effective barrier to 90.5 ± 2.9% of the nanoparticles. Further, our simulation suggests that a larger fraction of the 9.5 ± 2.9% of nanoparticles that travelled across the Caco-2/HT29-MTX cell layer were not large nanoparticle aggregates, but primarily single nanoparticles and small aggregates. After crossing the GI tract epithelium, nanoparticles that were administered in high doses estimated in terms of possible daily human consumption (240 and 480 × 1011 nanoparticles/mL) induced the release of aspartate aminotransferase (AST), an intracellular enzyme of the liver that indicates liver cell injury. Using the GI ‘tract – liver – other tissue’ system allowed us to observe compounding effects and detect liver tissue injury at lower nanoparticle concentrations than expected from experiments with liver tissue only. Our results indicate that body-on-a-chip devices are highly relevant in vitro models for evaluating nanoparticle interactions with human tissues.
Related Links	http://dx.doi.org/10.1039/c4lc00371c
Ending Page	3092
Page Count	12
Starting Page	3081
File Format	PDF
ISSN	14730189
e-ISSN	14730189
Journal	Lab on a chip
Issue Number	16
Volume Number	14
Language	English
Publisher Date	2014-08-21
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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