NDLI: Signal Propagation in Multi-Layer DNAzyme Cascades using Structured Chimeric Substrates **

Content Provider	PubMed Central
Author	Brown, Carl W. Lakin, Matthew R. Horwitz, Eli K. Fanning, M. Leigh West, Hannah E. Stefanovic, Darko Graves, Steven W.
Abstract	Signal propagation through enzyme cascades is a critical component of information processing in cellular systems. Although such systems have potential as biomolecular computing tools, rational design of synthetic protein networks remains infeasible. DNA strands with catalytic activity (DNAzymes) are an attractive alternative, enabling rational cascade design through predictable base-pair hybridization principles. Here we report multi-layered DNAzyme signaling and logic cascades. We achieve signaling between DNAzymes using a structured chimeric substrate (SCS) that releases a downstream activator after cleavage by an upstream DNAzyme. The SCS can be activated by various upstream DNAzymes, can be coupled to DNA strand displacement devices, and is highly resistant to interference from background DNA. This work enables rational design of synthetic DNAzyme regulatory networks, with potential applications in biomolecular computing, biodetection, and autonomous theranostics.
Related Links	http://dx.doi.org/10.1002/anie.201402691
Ending Page	7187
Page Count	5
Starting Page	7183
File Format	PDF
ISSN	14337851
e-ISSN	15213773
Journal	Angewandte Chemie (International ed. in English)
Issue Number	28
Volume Number	53
Language	English
Publisher Date	2014-07-07
Access Restriction	Open
Subject Keyword	Chemistry(all) Catalysis Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Catalysis

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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