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Nitro-fatty acids and cyclopentenone prostaglandins share strategies to activate the Keap1-Nrf2 system: a study using green fluorescent protein transgenic zebrafish
| Content Provider | PubMed Central |
|---|---|
| Author | Tsujita, Tadayuki Li, Li Nakajima, Hitomi Iwamoto, Noriko Nakajima-takagi, Yaeko Ohashi, Ken Kawakami, Koichi Kumagai, Yoshito Freeman, Bruce A. Yamamoto, Masayuki Kobayashi, Makoto |
| Copyright Year | 2010 |
| Abstract | Nitro-fatty acids are electrophilic fatty acids produced in vivo from nitrogen peroxide that have many physiological activities. We recently demonstrated that nitro-fatty acids activate the Keap1-Nrf2 system, which protects cells from damage owing to electrophilic or oxidative stresses via transactivating an array of cytoprotective genes, although the molecular mechanism how they activate Nrf2 is unclear. A number of chemical compounds with different structures have been reported to activate the Keap1-Nrf2 system, which can be categorized into at least six classes based on their sensing pathways. In this study, we showed that nitrooleic acid (OA-NO2), one of major nitro-fatty acids, activates Nrf2 in the same manner that of a cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) using transgenic zebrafish that expresses green fluorescent protein (GFP) in response to Nrf2 activators. In transgenic embryos, GFP was induced in the whole body by treatment with OA-NO2, 15d-PGJ2 or diethylmaleate (DEM), but not with hydrogen peroxide (H2O2), when exogenous Nrf2 and Keap1 were co-overexpressed. Induction by OA-NO2 or 15d-PGJ2 but not DEM was observed, even when a C151S mutation was introduced in Keap1. Our results support the contention that OA-NO2 and 15d-PGJ2 share an analogous cysteine code as electrophiles and also have similar anti-inflammatory roles. |
| Related Links | http://dx.doi.org/10.1111/j.1365-2443.2010.01466.x |
| Ending Page | 57 |
| Page Count | 12 |
| Starting Page | 46 |
| File Format | |
| ISSN | 13569597 |
| e-ISSN | 13652443 |
| Journal | Genes to cells : devoted to molecular & cellular mechanisms |
| Issue Number | 1 |
| Volume Number | 16 |
| Language | English |
| Publisher Date | 2011-01-01 |
| Access Restriction | Open |
| Subject Keyword | Medicine(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Genetics Medicine |
