NDLI: Drosophila lipid droplets buffer the H2Av supply to protect early embryonic development

Content Provider	PubMed Central
Author	Li, Zhihuan Johnson, Matthew R. Ke, Zhonghe Chen, Lili Welte, Michael A.
Copyright Year	2014
Abstract	Assembly of DNA into chromatin requires a delicate balancing act, as both dearth and excess of histones severely disrupts chromatin function [1–3]. In particular, cells need to carefully control histone stoichiometry: if different types of histones are incorporated into chromatin in an imbalanced manner, it can lead to altered gene expression, mitotic errors, and death [4–6]. Both the balance between individual core histones and between core histones and histone variants is critical [5, 7]. Here, we find that in early Drosophila embryos histone balance in the nuclei is regulated by lipid droplets, cytoplasmic fat storage organelles [8]. Lipid droplets were previously known to function in long-term histone storage: newly laid embryos contain large amounts of excess histones generated during oogenesis [9], and the maternal supplies of core histone H2A and the histone variant H2Av are anchored to lipid droplets via the novel protein Jabba [3]. We find that in these embryos synthesis of new H2A and H2Av is imbalanced and that newly produced H2Av can be recruited to lipid droplets. When droplet sequestration is disrupted by mutating Jabba, embryos display an elevated H2Av/H2A ratio in nuclei as well as mitotic defects, reduced viability, and hypersensitivity to H2Av overexpression. We propose that in Drosophila embryos lipid droplets serve as a histone buffer, not only storing maternal histones to support the early cell cycles, but also transiently sequestering H2Av produced in excess and thus ensuring proper histone balance in the nucleus.
Related Links	http://dx.doi.org/10.1016/j.cub.2014.05.022
Ending Page	1491
Page Count	7
Starting Page	1485
File Format	PDF
ISSN	09609822
e-ISSN	18790445
Journal	Current biology : CB
Issue Number	13
Volume Number	24
Language	English
Publisher Date	2014-07-01
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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