NDLI: A long PCR–based approach for DNA enrichment prior to next-generation sequencing for systematic studies 1

Content Provider	PubMed Central
Author	Simon, Uribe-convers Duke, Justin R. Moore, Michael J. Tank, David C.
Copyright Year	2014
Abstract	• Premise of the study: We present an alternative approach for molecular systematic studies that combines long PCR and next-generation sequencing. Our approach can be used to generate templates from any DNA source for next-generation sequencing. Here we test our approach by amplifying complete chloroplast genomes, and we present a set of 58 potentially universal primers for angiosperms to do so. Additionally, this approach is likely to be particularly useful for nuclear and mitochondrial regions.
Related Links	http://dx.doi.org/10.3732/apps.1300063
File Format	PDF
ISSN	21680450
e-ISSN	21680450
Journal	Applications in Plant Sciences
Issue Number	1
Volume Number	2
Language	English
Publisher	Botanical Society of America
Publisher Date	2014-01-01
Access Restriction	Open
Rights Holder	Botanical Society of America
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Plant Science Ecology, Evolution, Behavior and Systematics

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A long PCR–based approach for DNA enrichment prior to next-generation sequencing for systematic studies 1