NDLI: IL-12–induced IFN-γ is dependent on caspase-1 processing of the IL-18 precursor

Content Provider	PubMed Central
Author	Fantuzzi, Giamila Reed, David A. Dinarello, Charles A.
Copyright Year	1999
Abstract	IL-12 and IL-18 are IFN-γ–inducing cytokines. In the present study, the role of endogenous IL-18 in the induction of IFN-γ by IL-12 was investigated in mice. In the presence of a specific inhibitor of caspase-1 (also known as IL-1β–converting enzyme, or ICE) IL-12–induced IFN-γ from splenocytes was reduced by 85%. Using splenocytes from ICE-deficient mice, IL-12–induced IFN-γ was reduced by 80%. However, the role of ICE was not through processing and release of IL-1β. Neutralizing anti–IL-18 IgG reduced IL-12–induced IFN-γ in splenocytes by 85%. Splenocytes cultured in vitro spontaneously released IL-18 into the extracellular compartment over time. Extracellular levels of IL-18 significantly correlated with IL-12–induced IFN-γ and were reduced in cells obtained from ICE-deficient mice. In vivo, IL-12 administration increased circulating levels of IL-18 in wild-type mice but not in ICE-deficient mice. Both neutralization of IL-18 and ICE deficiency significantly reduced induction of circulating IFN-γ in mice receiving IL-12. The IL-18 precursor was constitutively expressed in the livers and spleens of untreated mice. Furthermore, administration of IL-12 significantly increased liver-associated IL-18 levels. These data demonstrate that endogenous, ICE-cleaved IL-18 significantly contributes to induction of IFN-γ by IL-12.
Related Links	http://dx.doi.org/10.1172/jci7501
Ending Page	767
Page Count	7
Starting Page	761
File Format	PDF
ISSN	00219738
Journal	Journal of Clinical Investigation
Issue Number	6
Volume Number	104
Language	English
Publisher	American Society for Clinical Investigation
Publisher Date	1999-09-15
Access Restriction	Open
Rights Holder	American Society for Clinical Investigation
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine

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