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| Content Provider | PubMed Central |
|---|---|
| Author | Wang, Pei-pei Xu, Du-juan Huang, Can Wang, Wei-ping Xu, Wen-ke |
| Copyright Year | 2014 |
| Abstract | Astragaloside is a saponin widely used in traditional Chinese medicine and has been reported to be a potent multidrug resistance (MDR) reversal agent. The present study investigated the role of astragaloside IV (ASIV) in the regulation of P-glycoprotein (P-gp, encoded by the mdr1 gene) and its effect on the reversal of MDR. The activity of ASIV was evaluated using human hepatic cancer cells Bel-7402 and the corresponding 5-fluorouracil (5-FU) resistant cells Bel-7402/FU. ASIV (0.08 mg/ml) potentiated the cytotoxicity of 5-FU which was demonstrated using the MTT assay on Bel-7402/FU cells. ASIV reduced the expression of P-gp as was revealed by immunocytochemistry. Accumulation and efflux studies with the P-gp substrate, rhodamine 123 (Rh123), demonstrated that ASIV inhibited P-gp-mediated drug efflux. Furthermore, it was demonstrated that ASIV enhanced the drug accumulation of 5-FU using a high performance liquid chromatography (HPLC) assay for drug resistant cells. Furthermore, ASIV may downregulate the expression of P-gp, which was examined using western blot analysis and polymerase chain reaction. In conclusion, the results of the present study indicated that ASIV reverses the drug resistance of Bel-7402/FU cells by downregulating the expression of mdr1. ASIV may represent a potent modulator of P-gp-mediated MDR in hepatic cancer therapy. |
| Related Links | http://dx.doi.org/10.3892/mmr.2014.2074 |
| Ending Page | 2137 |
| Page Count | 7 |
| Starting Page | 2131 |
| File Format | |
| ISSN | 17913004 |
| e-ISSN | 17913004 |
| Journal | Molecular Medicine Reports |
| Issue Number | 6 |
| Volume Number | 9 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2014-06-01 |
| Access Restriction | Open |
| Rights Holder | D.A. Spandidos |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
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