NDLI: Variant PRC1 Complex-Dependent H2A Ubiquitylation Drives PRC2 Recruitment and Polycomb Domain Formation

Content Provider	PubMed Central
Author	Blackledge, Neil P. Farcas, Anca M. Kondo, Takashi King, Hamish W. Mcgouran, Joanna F. Hanssen, Lars L. P. Ito, Shinsuke Cooper, Sarah Kondo, Kaori Koseki, Yoko Ishikura, Tomoyuki Long, Hannah K. Sheahan, Thomas W. Brockdorff, Neil Kessler, Benedikt M. Koseki, Haruhiko Klose, Robert J.
Copyright Year	2014
Abstract	Chromatin modifying activities inherent to polycomb repressive complexes PRC1 and PRC2 play an essential role in gene regulation, cellular differentiation, and development. However, the mechanisms by which these complexes recognize their target sites and function together to form repressive chromatin domains remain poorly understood. Recruitment of PRC1 to target sites has been proposed to occur through a hierarchical process, dependent on prior nucleation of PRC2 and placement of H3K27me3. Here, using a de novo targeting assay in mouse embryonic stem cells we unexpectedly discover that PRC1-dependent H2AK119ub1 leads to recruitment of PRC2 and H3K27me3 to effectively initiate a polycomb domain. This activity is restricted to variant PRC1 complexes, and genetic ablation experiments reveal that targeting of the variant PCGF1/PRC1 complex by KDM2B to CpG islands is required for normal polycomb domain formation and mouse development. These observations provide a surprising PRC1-dependent logic for PRC2 occupancy at target sites in vivo.
Related Links	http://dx.doi.org/10.1016/j.cell.2014.05.004
Ending Page	1459
Page Count	15
Starting Page	1445
File Format	PDF
ISSN	00928674
e-ISSN	10974172
Journal	Cell
Issue Number	6
Volume Number	157
Language	English
Publisher	Cell Press
Publisher Date	2014-06-01
Access Restriction	Open
Rights Holder	Cell Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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