NDLI: Exocyst-mediated membrane trafficking is required for branch outgrowth in Drosophila tracheal terminal cells

Content Provider	PubMed Central
Author	Jones, Tiffani A. Nikolova, Linda S. Schjelderup, Ani Metzstein, Mark M.
Abstract	Branching morphogenesis, the process by which cells or tissues generate tree-like networks that function to increases surface area or in contacting multiple targets, is a common developmental motif in multicellular organisms. We use Drosophila tracheal terminal cells, a component of the insect respiratory system, to investigate branching morphogenesis that occurs on the single cell level. Here, we show that the exocyst, a conserved protein complex that facilitates docking and tethering of vesicles at the plasma membrane, is required for terminal cell branch outgrowth. We find that exocyst-deficient terminal cells have highly truncated branches and show an accumulation of vesicles within their cytoplasm and are also defective in subcellular lumen formation. We also show that vesicle trafficking pathways mediated by the Rab GTPases Rab10 and Rab11 are redundantly required for branch outgrowth. In terminal cells, the PAR-polarity complex is required for branching, and we find the PAR complex is required for proper membrane localization of the exocyst, thus identifying a molecular link between the branching and outgrowth programs. Together, our results suggest a model where exocyst mediated vesicle trafficking facilitates branch outgrowth, while de novo branching requires cooperation between the PAR and exocyst complexes.
Related Links	http://dx.doi.org/10.1016/j.ydbio.2014.02.021
Ending Page	50
Page Count	10
Starting Page	41
File Format	PDF
ISSN	00121606
e-ISSN	1095564X
Journal	Developmental biology
Issue Number	1
Volume Number	390
Language	English
Publisher Date	2014-06-01
Access Restriction	Open
Subject Keyword	Developmental Biology Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Molecular Biology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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