NDLI: Purification and characterization of a guanine nucleotide-exchange protein for ADP-ribosylation factor from spleen cytosol.

Please wait, while we are loading the content...

Year: 2016, Volume: 113

Year: 2015, Volume: 112

Year: 2014, Volume: 111

Year: 2013, Volume: 110

Year: 2012, Volume: 109

Year: 2011, Volume: 109

Year: 2011, Volume: 108

Year: 2010, Volume: 107

Year: 2009, Volume: 106

Year: 2009, Volume: 94

Year: 2008, Volume: 105

Year: 2007, Volume: 104

Year: 2006, Volume: 103

Year: 2005, Volume: 103

Year: 2005, Volume: 102

Year: 2004, Volume: 101

Year: 2003, Volume: 100

Year: 2002, Volume: 99

Year: 2001, Volume: 98

Year: 2000, Volume: 97

Year: 1999, Volume: 96

Year: 1998, Volume: 95

Year: 1997, Volume: 94

Year: 1996, Volume: 93

Year: 1996, Volume: 93, Issue: 26

Year: 1996, Volume: 93, Issue: 25

Year: 1996, Volume: 93, Issue: 24

Year: 1996, Volume: 93, Issue: 23

Year: 1996, Volume: 93, Issue: 22

Year: 1996, Volume: 93, Issue: 21

Year: 1996, Volume: 93, Issue: 20

Year: 1996, Volume: 93, Issue: 19

Year: 1996, Volume: 93, Issue: 18

Year: 1996, Volume: 93, Issue: 17

Year: 1996, Volume: 93, Issue: 16

Year: 1996, Volume: 93, Issue: 15

Year: 1996, Volume: 93, Issue: 14

Year: 1996, Volume: 93, Issue: 13

Year: 1996, Volume: 93, Issue: 12

Year: 1996, Volume: 93, Issue: 11

Year: 1996, Volume: 93, Issue: 10

Year: 1996, Volume: 93, Issue: 9

Year: 1996, Volume: 93, Issue: 8

Year: 1996, Volume: 93, Issue: 7

Year: 1996, Volume: 93, Issue: 6

Year: 1996, Volume: 93, Issue: 5

Year: 1996, Volume: 93, Issue: 4

Year: 1996, Volume: 93, Issue: 3

Year: 1996, Volume: 93, Issue: 2

Year: 1996, Volume: 93, Issue: 1

Binding in the growth hormone receptor complex.

Interactions of protein antigens with antibodies.

Principles of protein-protein interactions.

Protein-protein interactions in the rigor actomyosin complex.

Functional implications of protein-protein interactions in icosahedral viruses.

Selective pressure of a quinoxaline nonnucleoside inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) on HIV-1 replication results in the emergence of nucleoside RT-inhibitor-specific (RT Leu-74-->Val or Ile and Val-75-->Leu or Ile) HIV-1 mutants.

Molecular cloning of a protein kinase whose phosphorylation is regulated by genetic adhesion during Chlamydomonas fertilization.

Determining RNA solution structure by segmental isotopic labeling and NMR: application to Caenorhabditis elegans spliced leader RNA 1.

Arachidonic and docosahexaenoic acids are biosynthesized from their 18-carbon precursors in human infants.

Hydrated myoglobin's anharmonic fluctuations are not primarily due to dihedral transitions.

A mean field model of ligand-protein interactions: implications for the structural assessment of human immunodeficiency virus type 1 protease complexes and receptor-specific binding.

High-level production of recombinant human lysosomal acid alpha-glucosidase in Chinese hamster ovary cells which targets to heart muscle and corrects glycogen accumulation in fibroblasts from patients with Pompe disease.

Binding of the sigma 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting.

Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli.

Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus.

DNA-protein binding assays from a single sea urchin egg: a high-sensitivity capillary electrophoresis method.

Yeast actin: polymerization kinetic studies of wild type and a poorly polymerizing mutant.

Association with capsid proteins promotes nuclear targeting of simian virus 40 DNA.

Receptor-mediated gene transfer into macrophages.

A two-subunit type I DNA topoisomerase (reverse gyrase) from an extreme hyperthermophile.

Vpr protein of human immunodeficiency virus type 1 forms cation-selective channels in planar lipid bilayers.

The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families.

Influence of alkyltransferase activity and chromosomal locus on mutational hotspots in Chinese hamster ovary cells.

Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens.

Identification, sequence, and expression of caveolin-2 defines a caveolin gene family.

Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers.

Cytoplasmic dynein is associated with slow axonal transport.

Embryonic stem cells express multiple Eph-subfamily receptor tyrosine kinases.

Platelet-derived growth factor activates protein kinase C epsilon through redundant and independent signaling pathways involving phospholipase C gamma or phosphatidylinositol 3-kinase.

Angiotensin II type 2 receptor mediates programmed cell death.

HLA-G expression during preimplantation human embryo development.

Evidence that two present-day components needed for the genetic code appeared after nucleated cells separated from eubacteria.

Structural variation of the pseudoautosomal region between and within inbred mouse strains.

Mutagenic specificity of solar UV light in nucleotide excision repair-deficient rodent cells.

Molecular phylogeny of the extinct ground sloth Mylodon darwinii.

Cloning and characterization of ERG25, the Saccharomyces cerevisiae gene encoding C-4 sterol methyl oxidase.

The basic motif-leucine zipper transcription factor Nrl can positively regulate rhodopsin gene expression.

A pre-Columbian Y chromosome-specific transition and its implications for human evolutionary history.

The estrogen receptor locus is associated with a major gene influencing litter size in pigs.

Increasing DNA repair methyltransferase levels via bone marrow stem cell transduction rescues mice from the toxic effects of 1,3-bis(2-chloroethyl)-1-nitrosourea, a chemotherapeutic alkylating agent.

Immunoregulatory properties of ISG15, an interferon-induced cytokine.

Development and characterization of a rodent model of immune-mediated cholangitis.

Differentiation of B cells in the nonlymphoid tissue of the synovial membrane of patients with rheumatoid arthritis.

Potent immunogenicity of the B subunits of Escherichia coli heat-labile enterotoxin: receptor binding is essential and induces differential modulation of lymphocyte subsets.

The molecular role of the common gamma c subunit in signal transduction reveals functional asymmetry within multimeric cytokine receptor complexes.

Expression of a single-chain HLA class I molecule in a human cell line: presentation of exogenous peptide and processed antigen to cytotoxic T lymphocytes.

Truncation of the class II beta-chain cytoplasmic domain influences the level of class II/invariant chain-derived peptide complexes.

Loss of high-affinity prostacyclin receptors in platelets and the lack of prostaglandin-induced inhibition of platelet-stimulated thrombin generation in subjects with spinal cord injury.

Isolation and characterization of a cell surface albumin-binding protein from vascular endothelial cells.

Molecular mechanisms of dexamethasone inhibition of nitric oxide synthase expression in interleukin 1 beta-stimulated mesangial cells: evidence for the involvement of transcriptional and posttranscriptional regulation.

Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation.

Induction of the WAF1/CIP1 protein and apoptosis in human T-cell leukemia virus type I-transformed lymphocytes after treatment with adriamycin by using a p53-independent pathway.

A Schistosoma mansoni fatty acid-binding protein, Sm14, is the potential basis of a dual-purpose anti-helminth vaccine.

Early p53 alterations in mouse skin carcinogenesis by UVB radiation: immunohistochemical detection of mutant p53 protein in clusters of preneoplastic epidermal cells.

The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches.

Expression cloning and functional characterization of the kidney cortex high-affinity proton-coupled peptide transporter.

Reversible visual hemineglect.

cAMP compartmentation is responsible for a local activation of cardiac Ca2+ channels by beta-adrenergic agonists.

Structure of the sodium channel pore revealed by serial cysteine mutagenesis.

Purification and characterization of a guanine nucleotide-exchange protein for ADP-ribosylation factor from spleen cytosol.

Antibodies against specific extracellular epitopes of the glucagon receptor block glucagon binding.

Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells.

Conscious recollection and the human hippocampal formation: evidence from positron emission tomography.

The transcriptional transactivator of simian foamy virus 1 binds to a DNA target element in the viral internal promoter.

A role of amphiphysin in synaptic vesicle endocytosis suggested by its binding to dynamin in nerve terminals.

Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction.

Bacteriophage lambda N protein alone can induce transcription antitermination in vitro.

Importance of the carboxyl-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system for phosphoryl donor specificity.

An inhibitor of p34cdc2/cyclin B that regulates the G2/M transition in Xenopus extracts.

Cloning and expression of a gene encoding an integrin-like protein in Candida albicans.

Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis.

Phosphorylation-dependent human immunodeficiency virus type 1 infection and nuclear targeting of viral DNA.

Bathorhodopsin structure in the room-temperature rhodopsin photosequence: picosecond time-resolved coherent anti-Stokes Raman scattering.

Phase separation in aqueous solutions of lens gamma-crystallins: special role of gamma s.

Inhibition of human immunodeficiency virus type 1 and vaccinia virus infection by a dominant negative factor of the interferon regulatory factor family expressed in monocytic cells.

Oral tolerance in myelin basic protein T-cell receptor transgenic mice: suppression of autoimmune encephalomyelitis and dose-dependent induction of regulatory cells.

X-ray structure of a vanadium-containing enzyme: chloroperoxidase from the fungus Curvularia inaequalis.

Suppression of experimental arthritis by gene transfer of interleukin 1 receptor antagonist cDNA.

Targeted disruption of gp130, a common signal transducer for the interleukin 6 family of cytokines, leads to myocardial and hematological disorders.

Isolation of an ovine pulmonary surfactant-associated anionic peptide bactericidal for Pasteurella haemolytica.

Codon repeats in genes associated with human diseases: fewer repeats in the genes of nonhuman primates and nucleotide substitutions concentrated at the sites of reiteration.

Single neuron control over a complex motor program.

Neuroprotective strategy for Alzheimer disease: intranasal administration of a fatty neuropeptide.

The peptide binding site of the substance P (NK-1) receptor localized by a photoreactive analogue of substance P: presence of a disulfide bond.

Structure-activity relationships derived by machine learning: the use of atoms and their bond connectivities to predict mutagenicity by inductive logic programming.

Myosin dynamics in live Dictyostelium cells.

The Drosophila melanogaster dodo (dod) gene, conserved in humans, is functionally interchangeable with the ESS1 cell division gene of Saccharomyces cerevisiae.

Monoclonal antibodies inhibit in vitro fibrillar aggregation of the Alzheimer beta-amyloid peptide.

Differential toxicity of mitomycin C and porfiromycin to aerobic and hypoxic Chinese hamster ovary cells overexpressing human NADPH:cytochrome c (P-450) reductase.

Construction and characterization of an azurin analog for the purple copper site in cytochrome c oxidase.

Retinoblastoma protein directly interacts with and activates the transcription factor NF-IL6.

Selective repression of transcriptional activators by Pbx1 does not require the homeodomain.

Activation of single whisker barrel in rat brain localized by functional magnetic resonance imaging.

Gain of chromosome 3q defines the transition from severe dysplasia to invasive carcinoma of the uterine cervix.

Longevity and the genetic determination of collagen glycoxidation kinetics in mammalian senescence.

Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination.

Cloning and characterization of a binding subunit of the interleukin 13 receptor that is also a component of the interleukin 4 receptor.

Identification, purification, and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe.

In vitro activation of the interferon-induced, double-stranded RNA-dependent protein kinase PKR by RNA from the 3' untranslated regions of human alpha-tropomyosin.

Inhibition of Plasmodium falciparum malaria using antisense oligodeoxynucleotides.

Bacterial cell division protein FtsZ assembles into protofilament sheets and minirings, structural homologs of tubulin polymers.

Down-regulation of NF-κB protein levels in activated human lymphocytes by 1,25-dihydroxyvitamin D3

Year: 1995, Volume: 92

Year: 1994, Volume: 91

Year: 1993, Volume: 90

Year: 1992, Volume: 89

Year: 1991, Volume: 88

Year: 1990, Volume: 87

Year: 1989, Volume: 86

Year: 1988, Volume: 85

Year: 1987, Volume: 84

Year: 1986, Volume: 83

Year: 1985, Volume: 82

Year: 1984, Volume: 81

Year: 1983, Volume: 80

Year: 1982, Volume: 79

Year: 1981, Volume: 78

Year: 1980, Volume: 77

Year: 1979, Volume: 76

Year: 1978, Volume: 75

Year: 1977, Volume: 74

Year: 1976, Volume: 73

Year: 1975, Volume: 72

Year: 1974, Volume: 71

Year: 1973, Volume: 70

Year: 1972, Volume: 69

Year: 1971, Volume: 68

Year: 1970, Volume: 67

Year: 1970, Volume: 66

Year: 1970, Volume: 65

Year: 1969, Volume: 64

Year: 1969, Volume: 63

Year: 1969, Volume: 62

Year: 1968, Volume: 61

Year: 1968, Volume: 60

Year: 1968, Volume: 59

Year: 1967, Volume: 58

Year: 1967, Volume: 57

Year: 1966, Volume: 56

Year: 1966, Volume: 55

Year: 1965, Volume: 54

Year: 1965, Volume: 53

Year: 1964, Volume: 52

Year: 1964, Volume: 51

Year: 1963, Volume: 50

Year: 1963, Volume: 49

Year: 1962, Volume: 48

Year: 1961, Volume: 47

Year: 1960, Volume: 46

Year: 1959, Volume: 45

Year: 1958, Volume: 44

Year: 1957, Volume: 43

Year: 1956, Volume: 42

Year: 1955, Volume: 41

Year: 1954, Volume: 40

Year: 1953, Volume: 39

Year: 1952, Volume: 38

Year: 1951, Volume: 37

Year: 1950, Volume: 36

Year: 1949, Volume: 35

Year: 1948, Volume: 34

Year: 1947, Volume: 33

Year: 1946, Volume: 32

Year: 1945, Volume: 31

Year: 1944, Volume: 30

Year: 1943, Volume: 29

Year: 1942, Volume: 28

Year: 1941, Volume: 27

Year: 1940, Volume: 26

Year: 1939, Volume: 25

Year: 1938, Volume: 24

Year: 1937, Volume: 23

Year: 1936, Volume: 22

Year: 1935, Volume: 21

Year: 1934, Volume: 20

Year: 1933, Volume: 19

Year: 1932, Volume: 18

Year: 1931, Volume: 17

Year: 1930, Volume: 16

Year: 1929, Volume: 15

Year: 1928, Volume: 14

Year: 1927, Volume: 13

Year: 1926, Volume: 12

Year: 1925, Volume: 11

Year: 1924, Volume: 10

Year: 1923, Volume: 9

Year: 1922, Volume: 8

Year: 1921, Volume: 7

Year: 1920, Volume: 6

Year: 1919, Volume: 5

Year: 1918, Volume: 4

Year: 1917, Volume: 3

Year: 1916, Volume: 2

Year: 1915, Volume: 1

Purification and characterization of a guanine nucleotide-exchange protein for ADP-ribosylation factor from spleen cytosol.

Content Provider	PubMed Central
Author	Tsai, S. C. Adamik, R. Moss, J. Vaughan, M.
Abstract	ADP-ribosylation factors (ARFs) are 20-kDa guanine nucleotide-binding proteins and are active in the GTP-bound state and inactive with GDP bound. ARF-GTP has a critical role in vesicular transport in several cellular compartments. Conversion of ARF-GDP to ARF-GTP is promoted by a guanine nucleotide-exchange protein (GEP). We earlier reported the isolation from bovine brain cytosol of a 700-kDa protein complex containing GEP activity that was inhibited by brefeldin A (BFA). Partial purification yielded an approximately 60-kDa BFA-insensitive GEP that enhanced binding of ARF1 and ARF3 to Golgi membranes. GEP has now been purified extensively from rat spleen cytosol in a BFA-insensitive, approximately 55-kDa form. It activated class I ARFs (ARFs 1 and 3) that were N-terminally myristoylated, but not nonmyristoylated ARFs from class-I, II, or III. GEP activity required MgCl2. In the presence of 0.6-0.8 mM MgCl2 and 1 mM EDTA, binding of guanosine 5'-[gamma[35S]thio]triphosphate ([35S]GTP gamma S) by ARF1 and ARF3 was equally high without and with GEP. At higher Mg2+ concentrations, binding without GEP was much lower; with 2-5 mM MgCl2, GEP-stimulated binding was maximal. The rate of GDP binding was much less than that of GTP gamma S with and without GEP. Phospholipids were necessary for GEP activity; phosphatidylinositol was more effective than phosphatidylserine, and phosphatidic acid was less so. Other phospholipids tested were ineffective. Maximal effects required approximately 200 microM phospholipid, with half-maximal activation at 15-20 microM. Release of bound [35S]GTP gamma S from ARF3 required the presence of both GEP and unlabeled GTP or GTP gamma S; GDP was much less effective. This characterization of the striking effects of Mg2+ concentration and specific phospholipids on the purified BFA-insensitive ARF GEP should facilitate experiments to define its function in vesicular transport.
Starting Page	305
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	93
Language	English
Publisher Date	1996-01-09
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in