NDLI: Vibrio cholerae-Induced Inflammation in the Neonatal Mouse Cholera Model

Content Provider	PubMed Central
Author	Bishop, Anne L. Bharathi, Patimalla Camilli, Andrew
Editor	Payne, S. M.
Copyright Year	2014
Abstract	Vibrio cholerae is the causative agent of the acute diarrheal disease of cholera. Innate immune responses to V. cholerae are not a major cause of cholera pathology, which is characterized by severe, watery diarrhea induced by the action of cholera toxin. Innate responses may, however, contribute to resolution of infection and must be required to initiate adaptive responses after natural infection and oral vaccination. Here we investigated whether a well-established infant mouse model of cholera can be used to observe an innate immune response. We also used a vaccination model in which immunized dams protect their pups from infection through breast milk antibodies to investigate innate immune responses after V. cholerae infection for pups suckled by an immune dam. At the peak of infection, we observed neutrophil recruitment accompanied by induction of KC, macrophage inflammatory protein 2 (MIP-2), NOS-2, interleukin-6 (IL-6), and IL-17a. Pups suckled by an immunized dam did not mount this response. Accessory toxins RtxA and HlyA played no discernible role in neutrophil recruitment in a wild-type background. The innate response to V. cholerae deleted for cholera toxin-encoding phage (CTXϕ) and part of rtxA was significantly reduced, suggesting a role for CTXϕ-carried genes or for RtxA in the absence of cholera toxin (CTX). Two extracellular V. cholerae DNases were not required for neutrophil recruitment, but DNase-deficient V. cholerae caused more clouds of DNA in the intestinal lumen, which appeared to be neutrophil extracellular traps (NETs), suggesting that V. cholerae DNases combat NETs. Thus, the infant mouse model has hitherto unrecognized utility for interrogating innate responses to V. cholerae infection.
Related Links	http://dx.doi.org/10.1128/iai.00054-14
Ending Page	2447
Page Count	14
Starting Page	2434
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	6
Volume Number	82
Language	English
Publisher	American Society for Microbiology
Publisher Date	2014-06-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cholera Caused by Vibrio cholerae O1 Induces T-Cell Responses in the Circulation▿

Vibrio cholerae hemagglutinin/protease nicks cholera enterotoxin.

Characterization of Vibrio cholerae Outer Membrane Vesicles as a Candidate Vaccine for Cholera▿

Vibrio cholerae-Induced Cellular Responses of Polarized T84 Intestinal Epithelial Cells Are Dependent on Production of Cholera Toxin and the RTX Toxin

Establishment of an Adult Mouse Model for Direct Evaluation of the Efficacy of Vaccines against Vibrio cholerae ▿

Development of a germfree mouse model of Vibrio cholerae infection.

Vibrio cholerae Intestinal Population Dynamics in the Suckling Mouse Model of Infection

Role of Vibrio cholerae neuraminidase in the function of cholera toxin.

Inhibition of cholera toxin production by thiols in Vibrio cholerae.

Vibrio cholerae-Induced Inflammation in the Neonatal Mouse Cholera Model

Similar Documents

Cholera Caused by Vibrio cholerae O1 Induces T-Cell Responses in the Circulation▿

Vibrio cholerae hemagglutinin/protease nicks cholera enterotoxin.

Characterization of Vibrio cholerae Outer Membrane Vesicles as a Candidate Vaccine for Cholera▿

Vibrio cholerae-Induced Cellular Responses of Polarized T84 Intestinal Epithelial Cells Are Dependent on Production of Cholera Toxin and the RTX Toxin

Establishment of an Adult Mouse Model for Direct Evaluation of the Efficacy of Vaccines against Vibrio cholerae ▿

Development of a germfree mouse model of Vibrio cholerae infection.

Vibrio cholerae Intestinal Population Dynamics in the Suckling Mouse Model of Infection

Role of Vibrio cholerae neuraminidase in the function of cholera toxin.

Inhibition of cholera toxin production by thiols in Vibrio cholerae.

Vibrio cholerae-Induced Inflammation in the Neonatal Mouse Cholera Model