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| Content Provider | PubMed Central |
|---|---|
| Author | West, Megan C. Campbell, Leah J. Willoughby, John J. Jensen, Abbie M. |
| Copyright Year | 2014 |
| Abstract | Temporal and spatial control of gene expression is important for studying the molecular and cellular mechanisms of development, physiology, and disease. We used the doxycycline (Dox)-inducible, Tet-On System to develop transgenic zebrafish for inducible, cell specific control of gene expression in the ultraviolet (UV) cone photoreceptors. Two constructs containing the reverse tetracycline-controlled transcriptional transactivator (rtTA) gene driven by the UV opsin-specific promoter (opn1sw1) were used to generate stable transgenic zebrafish lines using the Tol2-based transgenesis method. One construct included a self-reporting GFP (opn1sw1:rtTA, TRE:GFP) and the other incorporated an epitope tag on the rtTA protein (opn1sw1:rtTAflag ). UV cone-specific expression of TRE-controlled transgenes was induced by Dox treatment in larvae and adults. Induction of gene expression was observed in 96% of all larval UV cones within 16 hours of Dox treatment. UV cone-specific expression of two genes from a bidirectional TRE construct injected into one-cell Tg(opn1sw1:rtTAflag) embryos were also induced by Dox treatment. In addition, UV cone-specific expression of Crb2aIntraWT was induced by Dox treatment in progeny from crosses of the TRE-response transgenic line, Tg(TRE:HA-Crb2aIntraWT), to the Tg(opn1sw1:rtTA, TRE:GFP) line and the Tg(opn1sw1:rtTAflag) line. These lines can be used in addition to the inducible, rod-specific gene expression system from the Tet-On Toolkit to elucidate the photoreceptor-specific effects of genes of interest in photoreceptor cell biology and retinal disease. |
| Related Links | http://dx.doi.org/10.1016/j.gep.2014.01.002 |
| Ending Page | 104 |
| Page Count | 9 |
| Starting Page | 96 |
| File Format | |
| ISSN | 1567133X |
| e-ISSN | 18727298 |
| Journal | Gene expression patterns : GEP |
| Issue Number | 2 |
| Volume Number | 14 |
| Language | English |
| Publisher Date | 2014-03-01 |
| Access Restriction | Open |
| Subject Keyword | Genetics Developmental Neuroscience Molecular Biology Cellular and Molecular Neuroscience Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Developmental Biology Molecular Biology |
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