NDLI: Investigation of Bacterial Cellulose Biosynthesis Mechanism inGluconoacetobacter hansenii

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Content Provider	PubMed Central
Author	Mohite, Bhavna V. Patil, Satish V.
Copyright Year	2014
Abstract	The present study explores the mechanism of cellulose biosynthesis in Gluconoacetobacterhansenii. The cellulose synthase enzyme was purified as membrane fraction and solubilizedby treatment with 0.1% digitonin. The enzyme was separated by native-gel electrophoresis and β -D-glucan analysis was carried out using in vitro gelassay. The cellulose synthase has glycoprotein nature and composed two polypeptide subunits of93 KDa and 85 KDa. The confirmation of β -1,4-glucan (cellulose) was performed in whole andhydrolyzed monomeric sugar form. Tinopal and Congo red were used for cellulose detection on the gel.Thus the in vitro cellulose synthesis assay with cell free enzyme fraction was attempted to improvethe understanding of cellulose biosynthesis.
Related Links	http://dx.doi.org/10.1155/2014/836083
Starting Page	836083
File Format	PDF
ISSN	20907486
e-ISSN	20907486
Journal	ISRN Microbiology
Volume Number	2014
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2014-03-16
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article

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Investigation of Bacterial Cellulose Biosynthesis Mechanism inGluconoacetobacter hansenii