NDLI: Subcurative radiation significantly increases cell proliferation, invasion,and migration of primary glioblastoma multiforme in vivo

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Subcurative radiation significantly increases cell proliferation, invasion,and migration of primary glioblastoma multiforme in vivo

Content Provider	PubMed Central
Author	Shankar, Adarsh Kumar, Sanath Iskander, Asm Varma, Nadimpalli Rs Janic, Branislava Decarvalho, Ana Mikkelsen, Tom Frank, Joseph A. Ali, Meser M. Knight, Robert A. Brown, Stephen Arbab, Ali S.
Copyright Year	2014
Abstract	Tumor cell proliferation, infiltration, migration, and neovascularization are knowncauses of treatment resistance in glioblastoma multiforme (GBM). The purpose of this studywas to determine the effect of radiation on the growth characteristics of primary humanGBM developed in a nude rat. Primary GBM cells grown from explanted GBM tissues wereimplanted orthotopically in nude rats. Tumor growth was confirmed by magnetic resonanceimaging on day 77 (baseline) after implantation. The rats underwent irradiation to a doseof 50 Gy delivered subcuratively on day 84 postimplantation (n = 8), orunderwent no radiation (n = 8). Brain tissues were obtained on day 112(nonirradiated) or day 133 (irradiated). Immunohistochemistry was performed to determinetumor cell proliferation (Ki-67) and to assess the expression of infiltration marker(matrix metalloproteinase-2, MMP-2) and cell migration marker (CD44). Tumorneovascularization was assessed by microvessel density using von-Willebrand factor (vWF)staining. Magnetic resonance imaging showed well-developed, infiltrative tumors in 11weeks postimplantation. The proportion of Ki-67-positive cells in tumors undergoingradiation was (71 ± 15)% compared with (25 ± 12)% in the nonirradiated group(P = 0.02). The number of MMP-2-positive areas and proportion ofCD44-positive cells were also high in tumors receiving radiation, indicating greatinvasion and infiltration. Microvessel density analysis did not show a significantdifference between nonirradiated and irradiated tumors. Taken together, we found thatsubcurative radiation significantly increased proliferation, invasion, and migration ofprimary GBM. Our study provides insights into possible mechanisms of treatment resistancefollowing radiation therapy for GBM.
Related Links	http://dx.doi.org/10.5732/cjc.013.10095
Ending Page	158
Page Count	11
Starting Page	148
File Format	PDF
ISSN	1000467X
e-ISSN	1944446X
Journal	Chinese Journal of Cancer
Issue Number	3
Volume Number	33
Language	English
Publisher	Sun Yat-sen University Cancer Center
Publisher Date	2014-03-05
Access Restriction	Open
Rights Holder	Sun Yat-sen University Cancer Center
Subject Keyword	Oncology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Oncology

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