NDLI: Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

Content Provider	PubMed Central
Author	Prescott, Elizabeth M. Osheim, Yvonne N. Jones, Hannah S. Alen, Claudia M. Roan, Judith G. Reeder, Ronald H. Beyer, Ann L. Proudfoot, Nick J.
Copyright Year	2004
Abstract	We identify Rpa12p of RNA polymerase I (Pol I) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied to the rRNA-encoding genes of Saccharomyces cerevisiae. These confirm that Pol I termination occurs close to the Reb1p-dependent terminator in wild-type strains. However, deletion mutants for the 3′ end-processing enzyme Rnt1p or the Rpa12p subunit of Pol I both show Pol I transcription in the spacer. For Δrpa12, these spacer polymerases are devoid of nascent transcripts, suggesting they are immediately degraded. The homology of Rpa12p to the small subunit Rpb9p of Pol II and Rpc11p of Pol III, both implicated in transcriptional termination, points to a common termination mechanism for all three classes of RNA polymerase.
Related Links	http://dx.doi.org/10.1073/pnas.0401393101
Ending Page	6073
Page Count	6
Starting Page	6068
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	16
Volume Number	101
Language	English
Publisher	National Academy of Sciences
Publisher Date	2004-04-20
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Defining the Influence of the A12.2 Subunit on Transcription Elongation and Termination by RNA Polymerase I In Vivo

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RNA polymerase subunit RPB5 plays a role in transcriptional activation

Modulation of RNA polymerase II subunit composition by ubiquitylation

Termination of transcription by yeast RNA polymerase I.

Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

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