NDLI: A Computer-assisted Multi-electrode Patch-clamp System

Content Provider	PubMed Central
Author	Perin, Rodrigo Markram, Henry
Copyright Year	2013
Abstract	The patch-clamp technique is today the most well-established method for recording electrical activity from individual neurons or their subcellular compartments. Nevertheless, achieving stable recordings, even from individual cells, remains a time-consuming procedure of considerable complexity. Automation of many steps in conjunction with efficient information display can greatly assist experimentalists in performing a larger number of recordings with greater reliability and in less time. In order to achieve large-scale recordings we concluded the most efficient approach is not to fully automatize the process but to simplify the experimental steps and reduce the chances of human error while efficiently incorporating the experimenter's experience and visual feedback. With these goals in mind we developed a computer-assisted system which centralizes all the controls necessary for a multi-electrode patch-clamp experiment in a single interface, a commercially available wireless gamepad, while displaying experiment related information and guidance cues on the computer screen. Here we describe the different components of the system which allowed us to reduce the time required for achieving the recording configuration and substantially increase the chances of successfully recording large numbers of neurons simultaneously.
Related Links	http://dx.doi.org/10.3791/50630
Starting Page	50630
File Format	PDF
ISSN	1940087X
e-ISSN	1940087X
Journal	Journal of Visualized Experiments : JoVE
Issue Number	80
Language	English
Publisher	MyJove Corporation
Publisher Date	2013-10-18
Access Restriction	Open
Rights Holder	MyJove Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Biochemistry, Genetics and Molecular Biology Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

One-channel Cell-attached Patch-clamp Recording

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Methods for Patch Clamp Capacitance Recordings from the Calyx

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

A Computer-assisted Multi-electrode Patch-clamp System

Similar Documents

One-channel Cell-attached Patch-clamp Recording

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Methods for Patch Clamp Capacitance Recordings from the Calyx

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

A Computer-assisted Multi-electrode Patch-clamp System