NDLI: Re-Examining the Size/Charge Paradigm: Differing In Vivo Characteristics of Size and Charge-Matched Mesoporous Silica Nanoparticles

Content Provider	PubMed Central
Author	Townson, Jason L. Lin, Yu-shen Agola, Jacob O. Carnes, Eric C. Leong, Hon S. Lewis, John D. Haynes, Christy L. Brinker, C. Jeffrey
Abstract	The combination of nanoparticle (NP) size, charge, and surface chemistry (e.g. extent of modification with polyethylene glycol (PEG)) is accepted as a key determinant of NP/cellular interactions. However, the influence of spatial arrangement and accessibility of the charged molecules on the NP surface vis-à-vis the average surface charge (zeta potential) is incompletely understood. Here we demonstrate that two types of mesoporous silica nanoparticles (MSNP) that are matched in terms of primary and hydrodynamic particle size, shape, pore structure, colloidal stability, and zeta potential, but differ in surface chemistry, viz. the spatial arrangement and relative exposure of surface amines, have profoundly different interactions with cells and tissues when evaluated in vitro and in vivo. While both particles are ~50 nm in diameter, PEGylated, and positively charged (ζ = +40mV), PEG-PEI (MSNPs modified with exposed polyamines), but not PEG-NMe3 + (MSNP modified with distributed, obstructed amines) rapidly bind serum proteins, diverse cells types in vitro, and endothelial and white blood cells in vivo (ex ovo chick embryo model). This finding helps elucidate the relative role of surface exposure of charged molecules vs zeta potential in otherwise physicochemically matched MSNP and highlights protein corona neutrality as an important design consideration when synthesizing cationic NPs for biological applications.
Related Links	http://dx.doi.org/10.1021/ja4082414
Ending Page	16033
Page Count	4
Starting Page	16030
File Format	PDF
ISSN	15205126
e-ISSN	15205126
Journal	Journal of the American Chemical Society
Issue Number	43
Volume Number	135
Language	English
Publisher Date	2013-10-30
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Colloid and Surface Chemistry Biochemistry Catalysis

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bioresponsive Mesoporous Silica Nanoparticles for Triggered Drug Release

Highly Aminated Mesoporous Silica Nanoparticles with Cubic Pore Structure

Autonomous in Vitro Anticancer Drug Release from Mesoporous Silica Nanoparticles by pH-Sensitive Nanovalves

Photoexpulsion of Surface-Grafted Ruthenium Complexes and Subsequent Release of Cytotoxic Cargos to Cancer Cells from Mesoporous Silica Nanoparticles

Mesoporous Silica-Coated Hollow Manganese Oxide Nanoparticles as Positive T 1 Contrast Agents for Labeling and MRI Tracking of Adipose-Derived Mesenchymal Stem Cells

Activation of Snap-Top Capped Mesoporous Silica Nano Containers Using Two Near-Infrared Photons

Fidelity Discrimination in DNA polymerase β: differing closing profiles for a mismatched (G:A) versus matched (G:C) base pair

nm- thick conformal pore-sealing of self-assembled mesoporous silica by plasma-assisted atomic layer deposition

Engineered Biocompatible Nanoparticles for in Vivo Imaging Applications

Re-Examining the Size/Charge Paradigm: Differing In Vivo Characteristics of Size and Charge-Matched Mesoporous Silica Nanoparticles

Similar Documents

Bioresponsive Mesoporous Silica Nanoparticles for Triggered Drug Release

Highly Aminated Mesoporous Silica Nanoparticles with Cubic Pore Structure

Autonomous in Vitro Anticancer Drug Release from Mesoporous Silica Nanoparticles by pH-Sensitive Nanovalves

Photoexpulsion of Surface-Grafted Ruthenium Complexes and Subsequent Release of Cytotoxic Cargos to Cancer Cells from Mesoporous Silica Nanoparticles

Mesoporous Silica-Coated Hollow Manganese Oxide Nanoparticles as Positive T 1 Contrast Agents for Labeling and MRI Tracking of Adipose-Derived Mesenchymal Stem Cells

Activation of Snap-Top Capped Mesoporous Silica Nano Containers Using Two Near-Infrared Photons

Fidelity Discrimination in DNA polymerase β: differing closing profiles for a mismatched (G:A) versus matched (G:C) base pair

nm- thick conformal pore-sealing of self-assembled mesoporous silica by plasma-assisted atomic layer deposition

Engineered Biocompatible Nanoparticles for in Vivo Imaging Applications

Re-Examining the Size/Charge Paradigm: Differing In Vivo Characteristics of Size and Charge-Matched Mesoporous Silica Nanoparticles