NDLI: Stimulation and release from neurons via a dual capillary collection device interfaced to mass spectrometry

Content Provider	PubMed Central
Author	Fan, Yi Lee, Chang Young Rubakhin, Stanislav S. Sweedler, Jonathan V.
Abstract	Neuropeptides are cell to cell signaling molecules that modulate a wide range of physiological processes. Neuropeptide release has been studied in samples sizes ranging from single cells and neuronal clusters, to defined brain nuclei and large brain regions. We have developed and optimized cell stimulation and collection approaches for the efficient measurement of neuropeptide release from neuronal samples using a dual capillary system. The defining feature is a capillary that contains octadecyl-modified silica nanoparticles on its inner wall to capture and extract releasates. This collection capillary is inserted into another capillary used to deliver solutions that chemically stimulate the cells, with solution flow up the inner capillary to facilitate peptide collection. The efficiency of peptide collection was evaluated using six peptide standards mixed in physiological saline. The extracted peptides eluted from these capillaries were characterized via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with low femtomole detection limits. Using the capillary collection system in small custom-fabricated culturing chambers, individual cultured neurons and neuronal clusters from the model animal Aplysia californica were stimulated with distinct neuronal secretagogues and the releasates collected and characterized using MALDI-TOF MS.
Related Links	http://dx.doi.org/10.1039/c3an01010d
Ending Page	6346
Page Count	10
Starting Page	6337
File Format	PDF
ISSN	00032654
e-ISSN	13645528
Journal	The Analyst
Issue Number	21
Volume Number	138
Language	English
Publisher Date	2013-01-01
Access Restriction	Open
Subject Keyword	Analytical Chemistry Spectroscopy Electrochemistry Biochemistry Environmental Chemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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