NDLI: Purification and characterization of an extracellular laccase from solid-state culture of Pleurotus ostreatus HP-1

Please wait, while we are loading the content...

Application of extracellular lipopeptide biosurfactant produced by endophytic Bacillus subtilis K1 isolated from aerial roots of banyan (Ficus benghalensis) in microbially enhanced oil recovery (MEOR)

Establishment of cell suspension culture in Marchantia linearis Lehm & Lindenb. for the optimum production of flavonoids

Isolation and cloning of the endoglucanase gene from Bacillus pumilus and its expression in Deinococcus radiodurans

Graphene oxide from silk cocoon: a novel magnetic fluorophore for multi-photon imaging

Purification and characterization of an extracellular laccase from solid-state culture of Pleurotus ostreatus HP-1

Incidence of constitutive and inducible clindamycin resistance among hospital-associated Staphylococcus aureus

Genome subtraction for the identification of potential antimicrobial targets in Xanthomonas oryzae pv. oryzae PXO99A pathogenic to rice

Isolation of a multi-trait plant growth promoting Brevundimonas sp. and its effect on the growth of Bt-cotton

Acknowledgement to Referees

Year: 2013, Volume: 3

Year: 2012, Volume: 2

Year: 2011, Volume: 1

Purification and characterization of an extracellular laccase from solid-state culture of Pleurotus ostreatus HP-1

Content Provider	PubMed Central
Author	Patel, Hardik Gupte, Shilpa Mayur, Gahlout Gupte, Akshaya
Abstract	A native isolate of Pleurotus ostreatus HP-1 (Genbank Accession No. EU420068) was found to have an excellent laccase producing ability. The extracellular laccase was purified to electrophoretic homogeneity from copper sulphate induced solid-state fermentation medium by ammonium sulphate precipitation and ion-exchange chromatography. The enzyme was determined to be monomeric protein with an apparent molecular mass of 68,420 kDa, and an isoelectric point (pI) of 3.5. The inductively coupled plasma spectroscopy showed a presence of iron, zinc and copper in the purified enzyme. The absorption spectrum in the range of 200–700 nm showed the maximum absorption at 610 nm characteristic of fungal laccase and corresponding to the presence of type I copper atom. The laccase was stable at different temperatures up to 70 °C and retained 61 % activity at 50 °C. The enzyme reaction was inhibited by cysteine; sodium azide and EDTA. The enzyme oxidized various known laccase substrates, its lowest K m value being for ortho-dianisidine and highest K cat and K cat/K m for ABTS. The purified laccase exhibited different pH optima for different substrates. The N-terminal sequence did not show any similarity with N-terminal sequence of other species of genera Pleurotus.
Related Links	http://dx.doi.org/10.1007/s13205-013-0129-1
Ending Page	84
Page Count	8
Starting Page	77
File Format	PDF
ISSN	21905738
e-ISSN	21905738
Journal	3 Biotech
Issue Number	1
Volume Number	4
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-02-01
Access Restriction	Open
Rights Holder	Springer Berlin Heidelberg
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Environmental Science Agricultural and Biological Sciences Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Production purification and characterization of Laccase from Pleurotus ostreatus IIP 1 and its possible role in bioremediation of polycyclic aromatic hydrocarbons

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace

Purification, Characterization, and Functional Role of a Novel Extracellular Protease from Pleurotus ostreatus

Studies on laccase from Pleurotus ostreatus NCIM 1200 Production optimization under solid state fermentation characterization and application

Production optimization, purification, characterization and applications of Laccase secreted by Pleurotus ostreatus IMI 395545

Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization

Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

Degradation of aflatoxin $B_{1}$ from naturally contaminated maize using the edible fungus Pleurotus ostreatus springerOpen/

Biosurfactant production by Pleurotus ostreatus in submerged and solid-state fermentation systems

Purification and characterization of an extracellular laccase from solid-state culture of Pleurotus ostreatus HP-1

Similar Documents

Production purification and characterization of Laccase from Pleurotus ostreatus IIP 1 and its possible role in bioremediation of polycyclic aromatic hydrocarbons

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace

Purification, Characterization, and Functional Role of a Novel Extracellular Protease from Pleurotus ostreatus

Studies on laccase from Pleurotus ostreatus NCIM 1200 Production optimization under solid state fermentation characterization and application

Production optimization, purification, characterization and applications of Laccase secreted by Pleurotus ostreatus IMI 395545

Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization

Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

Degradation of aflatoxin $B_{1}$ from naturally contaminated maize using the edible fungus Pleurotus ostreatus springerOpen/

Biosurfactant production by Pleurotus ostreatus in submerged and solid-state fermentation systems

Purification and characterization of an extracellular laccase from solid-state culture of Pleurotus ostreatus HP-1