Loading...
Please wait, while we are loading the content...
Similar Documents
Cannabinoids Inhibit T-cells via Cannabinoid Receptor 2 in an in vitro Assay for Graft Rejection, the Mixed Lymphocyte Reaction
| Content Provider | PubMed Central |
|---|---|
| Author | Robinson, Rebecca Hartzell Meissler, Joseph J. Breslow-deckman, Jessica M. Gaughan, John Adler, Martin W. Eisenstein, Toby K. |
| Abstract | Cannabinoids are known to have anti-inflammatory and immunomodulatory properties. Cannabinoid receptor 2 (CB2) is expressed mainly on leukocytes and is the receptor implicated in mediating many of the effects of cannabinoids on immune processes. This study tested the capacity of Δ9-tetrahydrocannabinol (Δ9-THC) and of two CB2-selective agonists to inhibit the murine Mixed Lymphocyte Reaction (MLR), an in vitro correlate of graft rejection following skin and organ transplantation. Both CB2-selective agonists and Δ9-THC significantly suppressed the MLR in a dose dependent fashion. The inhibition was via CB2, as suppression could be blocked by pretreatment with a CB2-selective antagonist, but not by a CB1 antagonist, and none of the compounds suppressed the MLR when splenocytes from CB2 deficient mice were used. The CB2 agonists were shown to act directly on T-cells, as exposure of CD3+ cells to these compounds completely inhibited their action in a reconstituted MLR. Further, the CB2-selective agonists completely inhibited proliferation of purified T-cells activated by anti-CD3 and anti-CD28 antibodies. T-cell function was decreased by the CB2 agonists, as an ELISA of MLR culture supernatants revealed IL-2 release was significantly decreased in the cannabinoid treated cells. Together, these data support the potential of this class of compounds as useful therapies to prolong graft survival in transplant patients. |
| Related Links | http://dx.doi.org/10.1007/s11481-013-9485-1 |
| File Format | |
| ISSN | 15571904 |
| e-ISSN | 15571904 |
| Journal | Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology |
| Issue Number | 5 |
| Volume Number | 8 |
| Language | English |
| Publisher Date | 2013-12-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Immunology and Allergy Pharmacology Immunology |
