NDLI: Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection

Content Provider	PubMed Central
Author	Ahmad, Harith Thambiratnam, Kavintheran Zulkifli, Ahmad Z. Lawrence, Anthony Jasim, Ali A. Kunasekaran, Wijenthiran Musa, Sabri Gnanasegaran, Nareshwaran Vasanthan, Punitha Jayaraman, Pukana Kasim, Noor H. A. Govindasamy, Vijayendran Shahrir, Md. S. Harun, Sulaiman W.
Copyright Year	2013
Abstract	An efficient and low cost optical method for directly measuring the concentration of homogenous biological solutes is proposed and demonstrated. The proposed system operates by Fresnel reflection, with a flat-cleaved single-mode fiber serving as the sensor probe. A laser provides a 12.9 dBm sensor signal at 1,550 nm, while a computer-controlled optical power meter measures the power of the signal returned by the probe. Three different mesenchymal stem cell (MSC) lines were obtained, sub-cultured and trypsinized daily over 9 days. Counts were measured using a haemocytometer and the conditioned media (CM) was collected daily and stored at −80 °C. MSCs release excretory biomolecules proportional to their growth rate into the CM, which changes the refractive index of the latter. The sensor is capable of detecting changes in the number of stem cells via correlation to the change in the refractive index of the CM, with the measured power loss decreasing approximately 0.4 dB in the CM sample per average 1,000 cells in the MSC subculture. The proposed system is highly cost-effective, simple to deploy, operate, and maintain, is non-destructive, and allows reliable real-time measurement of various stem cell proliferation parameters.
Related Links	http://dx.doi.org/10.3390/s131013276
Ending Page	13288
Page Count	13
Starting Page	13276
File Format	PDF
ISSN	14248220
e-ISSN	14248220
Journal	Sensors (Basel, Switzerland)
Issue Number	10
Volume Number	13
Language	English
Publisher	Molecular Diversity Preservation International (MDPI)
Publisher Date	2013-09-01
Access Restriction	Open
Rights Holder	Molecular Diversity Preservation International (MDPI)
Subject Keyword	Electrical and Electronic Engineering Analytical Chemistry Atomic and Molecular Physics, and Optics Biochemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Atomic and Molecular Physics, and Optics Instrumentation Analytical Chemistry Biochemistry Information Systems Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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