NDLI: Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells

Please wait, while we are loading the content...

Antiretroviral Drug Interactions: Overview of Interactions Involving New and Investigational Agents and the Role of Therapeutic Drug Monitoring for Management

Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells

Encapsulation of Natural Polyphenolic Compounds; a Review

Formulation Optimization of Sustained-Release Ammonio Methacrylate Copolymer Microspheres. Effects of Log P and Concentration of Polar Cosolvents, and Role of the Drug/Copolymer Ratio

Exploring the Correlation Between Lipid Packaging in Lipoplexes and Their Transfection Efficacy

Pharmacokinetic Drug Interactions of Antimicrobial Drugs: A Systematic Review on Oxazolidinones, Rifamycines, Macrolides, Fluoroquinolones, and Beta-Lactams

Formulation Patents and Dermatology and Obviousness

Effect of Duration and Amplitude of Direct Current when Lidocaine Is Delivered by Iontophoresis

Ionic Channels as Targets for Drug Design: A Review on Computational Methods

Formulation and in Vitro, ex Vivo and in Vivo Evaluation of Elastic Liposomes for Transdermal Delivery of Ketorolac Tromethamine

Year: 2011, Volume: 3, Issue: 3

Year: 2011, Volume: 3, Issue: 2

Year: 2011, Volume: 3, Issue: 1

Year: 2010, Volume: 3

Year: 2010, Volume: 2

Year: 2009, Volume: 1

Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells

Content Provider	PubMed Central
Author	Peeters, Karen Wilmer, Martijn J. Schoeber, Joost P. Reijnders, Dorien Heuvel, Lambertus P. Van Den Masereeuw, Rosalinde Elena, Levtchenko
Copyright Year	2011
Abstract	P-glycoprotein (P-gp) is an ATP-dependent transporter localized at the apical membrane of the kidney proximal tubules, which plays a role in the efflux of cationic and amphipathic endogenous waste products and xenobiotics, such as drugs, into urine. Studies in mice deficient in P-gp showed generalized proximal tubular dysfunction similar to the phenotype of patients with cystinosis, an autosomal recessive disorder caused by mutations in the lysosomal cystine transporter cystinosin. Renal disease in cystinosis is characterized by generalized dysfunction of the apical proximal tubular influx transporters (so-called renal Fanconi syndrome) developing during infancy and gradually progressing towards end-stage renal disease before the 10th birthday in the majority of patients that are not treated with the cystine-depleting drug cysteamine. Here, we investigated whether the proximal tubular efflux transporter P-gp is affected in cystinosis and whether this might contribute to the development of renal Fanconi syndrome. We used conditionally immortalized (ci) proximal tubular epithelial cells (ciPTEC) derived from cystinotic patients and healthy volunteers. P-gp-mediated transport was measured by using the P-gp substrate calcein-AM in the presence and absence of the P-gp-inhibitor PSC833. P-gp activity was normal in cystinotic cells as compared to controls. Additionally, the effect of cysteamine on P-gp transport activity and phosphate uptake was determined; demonstrating increased P-gp activity in cystinotic cells, and further decrease of proximal tubular phosphate uptake. This observation is compatible with the persistence of renal Fanconi syndrome in vivo under cysteamine therapy. In summary, P-gp expression and activity are normal in cystinotic ciPTEC, indicating that P-gp dysfunction is not involved in the pathogenesis of cystinosis.
Related Links	http://dx.doi.org/10.3390/pharmaceutics3040782
Ending Page	792
Page Count	11
Starting Page	782
File Format	PDF
ISSN	19994923
e-ISSN	19994923
Journal	Pharmaceutics
Issue Number	4
Volume Number	3
Language	English
Publisher	MDPI
Publisher Date	2011-10-01
Access Restriction	Open
Rights Holder	MDPI
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Pharmaceutical Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells

P-Glycoprotein Induction Ameliorates Colistin Induced Nephrotoxicity in Cultured Human Proximal Tubular Cells

Role of serotonin in the regulation of renal proximal tubular epithelial cells

The role of HIF-1 in up-regulating MICA expression on human renal proximal tubular epithelial cells during hypoxia/reoxygenation

The MDR1 C3435T polymorphism: Effects on P-glycoprotein expression/function and clinical significance

High Glucose Increases Metallothionein Expression in Renal Proximal Tubular Epithelial Cells

The Effects of Cetirizine on P-glycoprotein Expression and Function In vitro and In situ

Is there a role for proximal tubular cells in regulating dendritic cell maturation and function in renal disease?

Costimulation with angiotensin II and interleukin 6 augments angiotensinogen expression in cultured human renal proximal tubular cells

Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells

Similar Documents

Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells

P-Glycoprotein Induction Ameliorates Colistin Induced Nephrotoxicity in Cultured Human Proximal Tubular Cells

Role of serotonin in the regulation of renal proximal tubular epithelial cells

The role of HIF-1 in up-regulating MICA expression on human renal proximal tubular epithelial cells during hypoxia/reoxygenation

The MDR1 C3435T polymorphism: Effects on P-glycoprotein expression/function and clinical significance

High Glucose Increases Metallothionein Expression in Renal Proximal Tubular Epithelial Cells

The Effects of Cetirizine on P-glycoprotein Expression and Function In vitro and In situ

Is there a role for proximal tubular cells in regulating dendritic cell maturation and function in renal disease?

Costimulation with angiotensin II and interleukin 6 augments angiotensinogen expression in cultured human renal proximal tubular cells

Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells