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Structural Mass Spectrometry Analysis of Lipid Changes in a Drosophila Epilepsy Model Brain
| Content Provider | PubMed Central |
|---|---|
| Author | Kliman, Michal Vijayakrishnan, Niranjana Wang, Lily Tapp, John T. Broadie, Kendal Mclean, John A. |
| Abstract | Phosphatidylethanolamine (PtdEtn) is one of the most abundant phospholipids in many animal cell types. The Drosophila easily shocked (eas 2) mutant, used as an epilepsy model, is null for the PtdEtn biosynthetic enzyme, ethanolamine kinase. This mutant displays bang sensitive paralysis, and was previously shown to have decreased levels of PtdEtn. We have developed a highly selective and sensitive measurement strategy using ion mobility-mass spectrometry for the relative quantitation of intact phospholipid species directly from isolated brain tissue of eas mutants. Over 1200 distinct lipid signals are observed and within this population 38, including PtdEtn, phosphatidylinositol (PtdIns) and phosphatidylcholine (PtdCho) species are identified to have changed significantly (p<0.03) between mutant and control tissue. This method has revealed for the first time the structural complexity and interconnectedness of specific PtdEtn and PtdIns lipid species within tissue, and provides great molecular detail compared to traditionally used detection techniques. |
| Related Links | http://dx.doi.org/10.1039/b927494d |
| File Format | |
| ISSN | 1742206X |
| e-ISSN | 17422051 |
| Journal | Molecular bioSystems |
| Issue Number | 6 |
| Volume Number | 6 |
| Language | English |
| Publisher Date | 2010-01-01 |
| Access Restriction | Open |
| Subject Keyword | Biotechnology Molecular Biology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biotechnology |
