NDLI: Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro

Content Provider	PubMed Central
Author	Endoh, Masaki Zhu, Wenyan Hasegawa, Jun Watanabe, Hajime Kim, Dong-ki Aida, Masatoshi Inukai, Naoto Narita, Takashi Yamada, Tomoko Furuya, Akiko Sato, Hiroe Yamaguchi, Yuki Mandal, Subhrangsu S. Reinberg, Danny Wada, Tadashi Handa, Hiroshi
Copyright Year	2004
Abstract	Recent studies have suggested that Spt6 participates in the regulation of transcription by RNA polymerase II (RNAPII). However, its underlying mechanism remains largely unknown. One possibility, which is supported by genetic and biochemical studies of Saccharomyces cerevisiae, is that Spt6 affects chromatin structure. Alternatively, Spt6 directly controls transcription by binding to the transcription machinery. In this study, we establish that human Spt6 (hSpt6) is a classic transcription elongation factor that enhances the rate of RNAPII elongation. hSpt6 is capable of stimulating transcription elongation both individually and in concert with DRB sensitivity-inducing factor (DSIF), comprising human Spt5 and human Spt4. We also provide evidence showing that hSpt6 interacts with RNAPII and DSIF in human cells. Thus, in vivo, hSpt6 may regulate multiple steps of mRNA synthesis through its interaction with histones, elongating RNAPII, and possibly other components of the transcription machinery.
Related Links	http://dx.doi.org/10.1128/mcb.24.8.3324-3336.2004
Ending Page	3336
Page Count	13
Starting Page	3324
File Format	PDF
ISSN	02707306
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	8
Volume Number	24
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-04-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro

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