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| Content Provider | PubMed Central |
|---|---|
| Author | Qu, Guanggang Fetterer, Raymond Jenkins, Mark Leng, Lin Richard, Bucala Shen, Zhiqiang Murphy, Charles Han, Wenyu Tuo, Wenbin |
| Abstract | The present study is the first characterization of Neospora caninum macrophage migration inhibitory factor (NcMIF). BLAST-N analysis of NcMIF revealed high similarity (87%) to the Toxoplasma gondii MIF. NcMIF was cloned and expressed in Escherichia coli in 3 forms, NcMIF (mature protein), NcMIFm (mutation of proline -2 to glycine), and NcMIFhis (addition of a polyhistidine tag at the N-terminus). None of these recombinant NcMIFs (rNcMIF) had tautomerase, oxidoreductase, or immunologic regulatory activities. rNcMIF was unable to compete with recombinant human MIF for a MIF receptor (CD74), suggesting that NcMIF does not bind to this MIF receptor. The glycine substitution for proline-2 of NcMIF resulted in increased retention time on SEC-HPLC and decreased formation of dimers and trimers. The addition of N-terminal HIS-tag led to increased formation of trimers. Immunofluorescence staining demonstrated that NcMIF was localized to the apical end of N. caninum tachyzoites. Immunoelectron microscopy further revealed that NcMIF was present in the micronemes, rhoptries, dense granules, and nuclei. NcMIF was abundant in the tachyzoite lysate and present in excretory and secretory antigen (ESAg) preparations. Total and secretory NcMIF was more (P<0.05) abundant in a non-pathologic clone, Ncts-8, than in the wild type isolate (NC1). Furthermore, NcMIF release by the both isolates was increased in the presence of calcium ionophore. This differential production of NcMIF by the pathologic and non-pathologic isolates of N. caninum may suggest a critical role of this molecule in the infectious pathogenesis of this parasite. |
| Related Links | http://dx.doi.org/10.1016/j.exppara.2013.07.001 |
| Ending Page | 256 |
| Page Count | 11 |
| Starting Page | 246 |
| File Format | |
| ISSN | 00144894 |
| e-ISSN | 10902449 |
| Journal | Experimental parasitology |
| Issue Number | 2 |
| Volume Number | 135 |
| Language | English |
| Publisher Date | 2013-10-01 |
| Access Restriction | Open |
| Subject Keyword | Immunology Parasitology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Parasitology Immunology |
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