NDLI: Transfusion of NOD mice with allogeneic newborn blood ameliorates autoimmune diabetes and modifies the expression of selected immune response genes 1, 2

Content Provider	PubMed Central
Author	Jayaraman, Sundararajan Patel, Tejas Patel, Vasu Ajani, Shahnaz Garza, Rebecca Jayaraman, Arathi Kwon, Sung Singh, Rajvir Rondelli, Damiano Prabhakar, Bellur S. Holterman, Mark
Abstract	Although allogeneic bone marrow transplantation has been shown to prevent autoimmune diabetes in heavily irradiated NOD mice, similar procedure is not suitable for the treatment of patients with type 1 diabetes because of associated severe side effects. Therefore, we evaluated whether mouse newborn blood, equivalent to human umbilical cord blood, could be used for diabetes prevention without recipient preconditioning. To test this hypothesis, unconditioned, pre-diabetic female NOD mice were given a single injection of whole newborn blood derived from the allogeneic, diabetes-resistant mouse strain, C57BL/6. Transfusion of allogeneic newborn but not adult blood prevented diabetes incidence in a majority of treated mice for a prolonged period of time. This was accompanied by the release of insulin in response to a challenge with glucose. Invasive cellular infiltration of islets was also substantially reduced in these mice. Although newborn blood transfusion induced low level of hematopoietic microchimerism, it did not strictly correlate with amelioration of diabetes. Induction of genes implicated in diabetes such as, Il18, Tnfa, and Inos but not Il4, Il17 or Ifng was repressed in splenocytes derived from protected mice. Notably, expression of the transcription factor Tbet/Tbx21 but not Gata3 or Rorgt was upregulated in protected mice. These data indicate that allogeneic newborn blood transfusion can prevent diabetes in NOD mice associated with modulation of selected cytokine genes implicated in diabetes manifestation. The data presented herein provide the proof of principle for the utility of allogeneic umbilical cord blood transfusion to treat patients with autoimmune diabetes.
Related Links	http://dx.doi.org/10.4049/jimmunol.0903615
Ending Page	3015
Page Count	8
Starting Page	3008
File Format	PDF
ISSN	00221767
e-ISSN	15506606
Journal	Journal of immunology (Baltimore, Md. : 1950)
Issue Number	6
Volume Number	184
Language	English
Publisher Date	2010-03-15
Access Restriction	Open
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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