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Primary spermatocyte-specific Cre recombinase activity in transgenic mice
| Content Provider | PubMed Central |
|---|---|
| Author | Chung, Sanny S. W. François, Cuzin Rassoulzadegan, Minoo Wolgemuth, Debra J. |
| Copyright Year | 2004 |
| Abstract | We have evaluated the specificity of Cre recombinase activity in transgenic mice expressing Cre under the control of the synatonemal complex protein 1 (Sycp1) gene promoter. Sycp1Cre mice were crossed with the ROSA26 reporter line R26R, to monitor the male germ cell stage-specificity of Cre activity as well as to verify that Cre was not active previously during development of other tissues. X-gal staining detected Cre-mediated recombination only in testis. Detailed histological examination indicated that weak Cre-mediated recombination occurred as early as in zygotene spermatocytes at stage XI of the cycle of the seminiferous epithelium. Robust expression of X-gal was detected in early to mid-late spermatocytes at stages V–VIII. We conclude that this transgenic line is a powerful tool for deleting genes of interest specifically during male meiosis. |
| Starting Page | 289 |
| File Format | |
| ISSN | 15739368 |
| e-ISSN | 15739368 |
| Journal | Transgenic research |
| Issue Number | 3 |
| Volume Number | 13 |
| Language | English |
| Publisher Date | 2004-06-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science |
