NDLI: Genetic characterization of moaB mutants of Escherichia coli

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Content Provider	PubMed Central
Author	Kozmin, Stanislav G. Schaaper, Roel M.
Abstract	The moaABCDE operon of Escherichia coli encodes enzymes essential for the biosynthesis of the molybdenum cofactor (Moco). However, the role of the moaB gene within this operon has remained enigmatic. Here, we have investigated the effect of moaB defects on two phenotypes diagnostic for Moco-deficiency: chlorate-resistance and sensitivity to the base analog 6-N-hydroxylaminopurine (HAP). We found that transposon insertions in moaB caused partial Moco-deficiency associated with chlorate-resistance, but not for HAP-sensitivity. On the other hand, in-frame deletions of moaB, or moaB overexpression, had no effect on either phenotype. Our combined data are consistent with the lack of any role for MoaB in Moco biosynthesis in E. coli.
Related Links	http://dx.doi.org/10.1016/j.resmic.2013.05.001
Ending Page	694
Page Count	6
Starting Page	689
File Format	PDF
ISSN	09232508
e-ISSN	17697123
Journal	Research in microbiology
Issue Number	7
Volume Number	164
Language	English
Publisher Date	2013-09-01
Access Restriction	Open
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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Genetic characterization of moaB mutants of Escherichia coli