NDLI: A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability

Content Provider	PubMed Central
Author	Regonesi, Maria Elena Briani, Federica Andrea, Ghetta Sandro, Zangrossi Daniela, Ghisotti Tortora, Paolo Gianni, Dehò
Copyright Year	2004
Abstract	Polynucleotide phosphorylase (PNPase), a 3′ to 5′ exonuclease encoded by pnp, plays a key role in Escherichia coli RNA decay. The enzyme, made of three identical 711 amino acid subunits, may also be assembled in the RNA degradosome, a heteromultimeric complex involved in RNA degradation. PNPase autogenously regulates its expression by promoting the decay of pnp mRNA, supposedly by binding at the 5′-untranslated leader region of an RNase III-processed form of this transcript. The KH and S1 RNA-binding domains at the C-terminus of the protein (amino acids 552–711) are thought to be involved in pnp mRNA recognition. Here we show that a G454D substitution in E.coli PNPase impairs autogenous regulation whereas it does not affect the catalytic activities of the enzyme. Although the mutation maps outside of the KH and S1 RNA-binding domains, analysis of the mutant protein revealed a defective RNA binding, thus suggesting that other determinants may be involved in PNPase–RNA interactions. The mutation also caused a looser association with the degradosome and an abnormal electrophoretic mobility in native gels. The latter feature suggests an altered structural conformation of PNPase, which may account for the properties of the mutant protein.
Related Links	http://dx.doi.org/10.1093/nar/gkh268
Ending Page	1017
Page Count	12
Starting Page	1006
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	3
Volume Number	32
Language	English
Publisher	Oxford University Press
Publisher Date	2004-02-13
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability

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A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability

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A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability

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A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability