NDLI: Sphingolipid Metabolites Modulate Dielectric Characteristics of Cells in a MouseOvarian Cancer Progression Model

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Sphingolipid Metabolites Modulate Dielectric Characteristics of Cells in a MouseOvarian Cancer Progression Model

Content Provider	PubMed Central
Author	Salmanzadeh, Alireza Elvington, Elizabeth S. Roberts, Paul C. Schmelz, Eva M. Davalos, Rafael V.
Abstract	Currently, conventional cancer treatment regimens often rely upon highly toxicchemotherapeutics or target oncogenes that are variably expressed within the heterogeneous cellpopulation of tumors. These challenges highlight the need for novel treatment strategies that 1) arenon-toxic yet able to at least partially reverse the aggressive phenotype of the disease to a benignor very slow-growing state, and 2) act on the cells independently of variably expressed biomarkers.Using a label-independent rapid microfluidic cell manipulation strategy known as contactlessdielectrophoresis (cDEP), we investigated the effect of non-toxic concentrations of two bioactivesphingolipid metabolites, sphingosine (So), with potential anti-tumor properties, andsphingosine-1-phosphate (S1P), a tumor-promoting metabolite, on the intrinsic electrical propertiesof early and late stages of mouse ovarian surface epithelial (MOSE) cancer cells. Previously, wedemonstrated that electrical properties change as cells progress from a benign early stage to latemalignant stages. Here, we demonstrate an association between So treatment and a shift in thebioelectrical characteristics of late stage MOSE (MOSE-L) cells towards a profile similar to that ofbenign MOSE-E cells. Particularly, the specific membrane capacitance of MOSE-L cells shifted towardthat of MOSE-E cells, decreasing from 23.94±2.75 to 16.46±0.62 mF/m2after So treatment, associated with a decrease in membrane protrusions. In contrast, S1P did notreverse the electrical properties of MOSE-L cells. This work is the first to indicate that treatmentwith non-toxic doses of So correlates with changes in the electrical properties and surfaceroughness of cells. It also demonstrates the potential of cDEP to be used as a new, rapid techniquefor drug efficacy studies, and eventually designing more personalized treatment regimens.
Related Links	http://dx.doi.org/10.1039/c3ib00008g
Ending Page	852
Page Count	10
Starting Page	843
File Format	PDF
ISSN	17579708
e-ISSN	17579708
Journal	Integrative biology : quantitative biosciences from nano to macro
Issue Number	6
Volume Number	5
Language	English
Publisher Date	2013-06-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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