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| Content Provider | PubMed Central |
|---|---|
| Author | Raphael, Itay Swetha, Mahesula Karan, Kalsaria Venkat, Kotagiri Purkar, Anjali B. Anjanappa, Manjushree Shah, Darshit Vidya, Pericherla Jadhav, Yeshwant Lal Avinash Raghunathan, Rekha Michael, Vaynberg Noriega, David Grimaldo, Nazul H. Wenk, Carola Gelfond, Jonathan A. L. Forsthuber, Thomas G. Haskins, William E. |
| Copyright Year | 2012 |
| Abstract | We hypothesized that quantitative MS/MS-based proteomics at multiple time points, incorporating rapid microwave and magnetic (M2) sample preparation, could enable relative protein expression to be correlated to disease progression in the experimental autoimmune encephalomyelitis (EAE) animal model of multiple sclerosis. To test our hypothesis, microwave-assisted reduction/alkylation/digestion of proteins from brain tissue lysates bound to C8 magnetic beads and microwave-assisted isobaric chemical labeling were performed of released peptides, in 90 s prior to unbiased proteomic analysis. Disease progression in EAE was assessed by scoring clinical EAE disease severity and confirmed by histopathologic evaluation for central nervous system inflammation. Decoding the expression of 283 top-ranked proteins (p <0.05) at each time point relative to their expression at the peak of disease, from a total of 1191 proteins observed in four technical replicates, revealed a strong statistical correlation to EAE disease score, particularly for the following four proteins that closely mirror disease progression: 14-3-3ε (p = 3.4E-6); GPI (p = 2.1E-5); PLP1 (p = 8.0E-4); PRX1 (p = 1.7E-4). These results were confirmed by Western blotting, signaling pathway analysis, and hierarchical clustering of EAE risk groups. While validation in a larger cohort is underway, we conclude that M2 proteomics is a rapid method to quantify putative prognostic/predictive protein biomarkers and therapeutic targets of disease progression in the EAE animal model of multiple sclerosis. |
| Related Links | http://dx.doi.org/10.1002/elps.201200200 |
| Ending Page | 3819 |
| Page Count | 10 |
| Starting Page | 3810 |
| File Format | |
| ISSN | 15222683 |
| e-ISSN | 15222683 |
| Journal | Electrophoresis |
| Issue Number | 24 |
| Volume Number | 33 |
| Language | English |
| Publisher Date | 2012-12-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Clinical Biochemistry Biochemistry |
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