NDLI: Sequence Analysis of In Vivo Defective Interfering-Like RNA of Influenza A H1N1 Pandemic Virus

Content Provider	PubMed Central
Author	Saira, Kazima Lin, Xudong Depasse, Jay V. Halpin, Rebecca Twaddle, Alan Stockwell, Timothy Angus, Brian Alessandro, Cozzi-lepri Delfino, Marina Dugan, Vivien Dwyer, Dominic E. Freiberg, Matthew Horban, Andrzej Marcelo, Losso Ruth, Lynfield Wentworth, Deborah N. Holmes, Edward C. Davey, Richard Wentworth, David E. Ghedin, Elodie
Copyright Year	2013
Abstract	Influenza virus defective interfering (DI) particles are naturally occurring noninfectious virions typically generated during in vitro serial passages in cell culture of the virus at a high multiplicity of infection. DI particles are recognized for the role they play in inhibiting viral replication and for the impact they have on the production of infectious virions. To date, influenza virus DI particles have been reported primarily as a phenomenon of cell culture and in experimentally infected embryonated chicken eggs. They have also been isolated from a respiratory infection of chickens. Using a sequencing approach, we characterize several subgenomic viral RNAs from human nasopharyngeal specimens infected with the influenza A(H1N1)pdm09 virus. The distribution of these in vivo-derived DI-like RNAs was similar to that of in vitro DIs, with the majority of the defective RNAs generated from the PB2 (segment 1) of the polymerase complex, followed by PB1 and PA. The lengths of the in vivo-derived DI-like segments also are similar to those of known in vitro DIs, and the in vivo-derived DI-like segments share internal deletions of the same segments. The presence of identical DI-like RNAs in patients linked by direct contact is compatible with transmission between them. The functional role of DI-like RNAs in natural infections remains to be established.
Related Links	http://dx.doi.org/10.1128/jvi.00240-13
Ending Page	8074
Page Count	11
Starting Page	8064
File Format	PDF
ISSN	0022538X
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	14
Volume Number	87
Language	English
Publisher	American Society for Microbiology
Publisher Date	2013-07-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Virology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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