NDLI: Droplet microfluidics for amplification-free genetic detection of single cells

Content Provider	PubMed Central
Author	Rane, Tushar D. Zec, Helena Puleo, Chris Lee, Abraham P. Wang, Tza-huei
Abstract	In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect 16S rRNA present in pathogenic cells. Initially the sensitivity and quantification ability of the platform is tested using a synthetic target mimicking the actual expression level of 16S rRNA in single cells. The capability of the device to perform “sample-to-answer” pathogen detection of single cells is demonstrated using E. coli as a model pathogen.
Related Links	http://dx.doi.org/10.1039/c2lc40537g
Starting Page	3341
File Format	PDF
ISSN	14730197
e-ISSN	14730189
Journal	Lab on a chip
Issue Number	18
Volume Number	12
Language	English
Publisher Date	2012-01-01
Access Restriction	Open
Subject Keyword	Biochemistry Bioengineering Chemistry(all) Biomedical Engineering Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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Droplet microfluidics for amplification-free genetic detection of single cells