NDLI: Quantitative orientation-independent DIC microscope with fast switching shear direction and bias modulation

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Quantitative orientation-independent DIC microscope with fast switching shear direction and bias modulation

Content Provider	PubMed Central
Author	Michael, Shribak
Abstract	We describe quantitative orientation-independent differential interference contrast (OI-DIC) microscope, which allows the bias retardation to be modulated and shear directions to be switched rapidly without any mechanically moving. The shear direction is switched by regular liquid crystal cell sandwiched between two standard DIC prisms. Another liquid crystal cell modulates the bias. Techniques for measuring parameters of DIC prisms and calibrating the bias are shown. Two sets of raw DIC images with the orthogonal shear directions are captured within a second. Then the quantitative image of optical path gradient distribution within a thin optical section is computed. The gradient data are used to obtain quantitative distribution of optical path, which represents refractive index gradient or height distribution. Computing enhanced regular DIC images with any desired shear direction is also possible.
Starting Page	769
File Format	PDF
ISSN	15208532
e-ISSN	15208532
Journal	Journal of the Optical Society of America. A, Optics, image science, and vision
Issue Number	4
Volume Number	30
Language	English
Publisher Date	2013-04-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Atomic and Molecular Physics, and Optics Electronic, Optical and Magnetic Materials Computer Vision and Pattern Recognition

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