NDLI: High Fidelity of Yellow Fever Virus RNA Polymerase

Content Provider	PubMed Central
Author	Pugachev, Konstantin V. Guirakhoo, Farshad Ocran, Simeon W. Mitchell, Fred Parsons, Megan Caroline, Penal Girakhoo, Soheila Pougatcheva, Svetlana O. Arroyo, Juan Trent, Dennis W. Monath, Thomas P.
Copyright Year	2004
Abstract	Three consecutive plaque purifications of four chimeric yellow fever virus-dengue virus (ChimeriVax-DEN) vaccine candidates against dengue virus types 1 to 4 were performed. The genome of each candidate was sequenced by the consensus approach after plaque purification and additional passages in cell culture. Our data suggest that the nucleotide sequence error rate for SP6 RNA polymerase used in the in vitro transcription step to initiate virus replication was as high as 1.34 × 10−4 per copied nucleotide and that the error rate of the yellow fever virus RNA polymerase employed by the chimeras for genome replication in infected cells was as low as 1.9 × 10−7 to 2.3 × 10−7. Clustering of beneficial mutations that accumulated after multiple virus passages suggests that the N-terminal part of the prM protein, a specific site in the middle of the E protein, and the NS4B protein may be essential for nucleocapsid-envelope interaction during flavivirus assembly.
Related Links	http://dx.doi.org/10.1128/jvi.78.2.1032-1038.2004
Ending Page	1038
Page Count	7
Starting Page	1032
File Format	PDF
ISSN	0022538X
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	2
Volume Number	78
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-01-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Virology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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