NDLI: Examination of the Subsarcolemmal Tubular System of Mammalian Skeletal Muscle Fibers

Content Provider	PubMed Central
Author	Jayasinghe, Isuru D. Lo, Harriet P. Morgan, Garry P. Baddeley, David Parton, Robert G. Christian, Soeller Launikonis, Bradley S.
Copyright Year	2013
Abstract	A subsarcolemmal tubular system network (SSTN) has been detected in skeletal muscle fibers by confocal imaging after the removal of the sarcolemma. Here we confirm the existence and resolve the fine architecture and the localization of the SSTN at an unprecedented level of detail by examining extracellularly applied tubular system markers in skeletal muscle fiber preparations with a combination of three imaging modalities: confocal fluorescence microscopy, direct stochastic optical reconstruction microscopy, and tomographic electron microscopy. Three-dimensional reconstructions showed that the SSTN was a dense two-dimensional network within the subsarcolemmal space around the fiber, running ∼500–600 nm underneath and parallel to the sarcolemma. The SSTN is composed of tubules ∼95 nm in width with ∼60% of the tubules directed transversely and >30% directed longitudinally. The deeper regular transverse tubules located at each A-I boundary of the sarcomeres branched from the SSTN, indicating individual transverse tubules that form triads are continuous with, but do not directly contact the sarcolemma. This suggests that the SSTN plays an important role in affecting the exchange of deeper tubule lumina with the extracellular space.
Related Links	http://dx.doi.org/10.1016/j.bpj.2013.04.029
Starting Page	19
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	11
Volume Number	104
Language	English
Publisher	The Biophysical Society
Publisher Date	2013-06-01
Access Restriction	Open
Rights Holder	The Biophysical Society
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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