NDLI: Formation of the transcription initiation complex on mammalian rDNA.

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Transcriptional analysis of Ty1 deletion and inversion derivatives at CYC7.

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Identification of the crossover site during FLP-mediated recombination in the Saccharomyces cerevisiae plasmid 2 microns circle.

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Expression of a secreted transplantation antigen gene during murine embryogenesis.

Repair of heteroduplex plasmid DNA after transformation into Saccharomyces cerevisiae.

Activated v-myc and v-ras oncogenes do not transform normal human lymphocytes.

Formation of the transcription initiation complex on mammalian rDNA.

Repair-deficient xeroderma pigmentosum cells made UV light resistant by fusion with X-ray-inactivated Chinese hamster cells.

Isolation and characterization of expressible cDNA clones encoding the M1 and M2 subunits of mouse ribonucleotide reductase.

Pyrimidine dimers block simian virus 40 replication forks.

Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.

Induction, by thymidylate stress, of genetic recombination as evidenced by deletion of a transferred genetic marker in mouse FM3A cells.

Identification of separate domains in the adenovirus E1A gene for immortalization activity and the activation of virus early genes.

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Saccharomyces cerevisiae nuclear fusion requires prior activation by alpha factor.

Induced change in a non-mendelian determinant by transplantation of macronucleoplasm in Paramecium tetraurelia.

Amino-terminal fragments of delta 1-pyrroline-5-carboxylate dehydrogenase direct beta-galactosidase to the mitochondrial matrix in Saccharomyces cerevisiae.

Bidirectional promoter elements of simian virus 40 are required for efficient replication of the viral DNA.

Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe.

Overproduction of protein p53 contributes to simian virus 40-mediated transformation.

Expression of c-sis and platelet-derived growth factor in in vitro-transformed glioma cells from rat brain tissue transplacentally treated with ethylnitrosourea.

Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media.

Coinfection with viruses carrying the v-Ha-ras and v-myc oncogenes leads to growth factor independence by an indirect mechanism.

A transcriptional enhancer and an interferon-responsive sequence in major histocompatibility complex class I genes.

Excision repair functions in Saccharomyces cerevisiae recognize and repair methylation of adenine by the Escherichia coli dam gene.

Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae.

Tissue-specific expression of the rat alpha 2u globulin gene family.

Intramolecular Recombination between Transfected Repeated Sequences in Mammalian Cells Is Nonconservative

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Formation of the transcription initiation complex on mammalian rDNA.

Content Provider	PubMed Central
Author	Kato, H. Nagamine, M. Kominami, R. Muramatsu, M.
Abstract	Steps for the formation of transcription initiation complex on the human rRNA gene (rDNA) in vitro were analyzed with partially purified transcription factors and RNA polymerase I. The reaction requires at least two factors besides RNA polymerase I for maximal efficiency. Preincubation and short-pulse analyses of the accurate transcripts revealed the following steps. First, the species-dependent factor, designated TFID, bound to the rDNA template, forming a preinitiation complex (PIC-1) which was resistant to a moderate concentration (0.015 to 0.02%) of Sarkosyl. Other factors, designated TFIA and RNA polymerase I, were then added to convert it to the final preinitiation complex PIC-3. This complex incorporated the first two nucleoside triphosphates of the starting site to complete the initiation complex (IC), which was resistant to a high concentration (0.2%) of Sarkosyl. Binding of TFID was rate limiting in the overall initiation reaction in vitro. Together with the kinetics of incorporation, the results are interpreted to mean that TFID, one bound, remains complexed with rDNA together with TFIA as the PIC-2 for many rounds of transcription by RNA polymerase I. Thus, the formation of PIC-2 may be a prerequisite for the stable opening of rDNA for transcription in vivo.
Starting Page	3418
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	10
Volume Number	6
Language	English
Publisher Date	1986-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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