NDLI: Unstable expression and amplification of a transfected oncogene in confluent and subconfluent cells.

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trans Activation of the simian virus 40 late transcription unit by T-antigen.

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Incorporation of a charged amino acid into the membrane-spanning domain blocks cell surface transport but not membrane anchoring of a viral glycoprotein.

Biochemical properties of a highly purified v-rasH p21 protein overproduced in Escherichia coli and inhibition of its activities by a monoclonal antibody.

Unstable expression and amplification of a transfected oncogene in confluent and subconfluent cells.

Structure of the promoter of the Dictyostelium discoideum prespore EB4 gene.

Structure of the Dictyostelium discoideum prestalk D11 gene and protein.

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Linker scanning mutagenesis of the 5'-flanking region of the mouse beta-major-globin gene: sequence requirements for transcription in erythroid and nonerythroid cells.

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DNase I-hypersensitive sites in the galactose gene cluster of Saccharomyces cerevisiae.

Sequences in human cytomegalovirus which hybridize with the avian retrovirus oncogene v-myc are G + C rich and do not hybridize with the human c-myc gene.

Induction of cellular DNA synthesis by a simian virus 40 mutant defective in nuclear transport of T antigen.

Host species specificity of polyomavirus DNA replication is not altered by simian virus 40 72-base-pair repeats.

High-resolution mapping of S1- and DNase I-hypersensitive sites in chromatin.

Expression of ras cellular oncogenes during development of Drosophila melanogaster.

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Unstable expression and amplification of a transfected oncogene in confluent and subconfluent cells.

Content Provider	PubMed Central
Author	Glanville, N.
Abstract	NIH 3T3 cells were transfected with a plasmid containing the transforming gene, v-src, from Rous sarcoma virus. One of the transformed cell lines isolated reverted to a flat, nontransformed morphology after cloning through soft agar. This cell line did not express the src gene and could no longer grow in soft agar. When these cells were held at confluence, spontaneous foci appeared which eventually covered the dish. The appearance of foci correlated with an increase in v-src gene expression, ability to grow in soft agar, and tumorigenicity in mice. When these transformed cells were trypsinized and held at subconfluence, both v-src expression and the transformed phenotype were progressively lost. Whereas rearrangement of the transfected gene was not detected, the gene copy number in the transformed cells was markedly increased (greater than 50-fold). Confluence-dependent gene amplification and deamplification have been retained after several cycles of growth alternately at high and low density, in cells recloned through soft agar, and after cells had been maintained continuously at high or low density. The results suggest that, in this cell line, reversible gene amplification plays a central role in expression of the transfected gene.
Starting Page	1456
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	6
Volume Number	5
Language	English
Publisher Date	1985-06-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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