NDLI: Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells.

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Characterization of an essential Saccharomyces cerevisiae gene related to RNA processing: cloning of RNA1 and generation of a new allele with a novel phenotype.

Characterization of pp60src phosphorylation in vitro in Rous sarcoma virus-transformed cell membranes.

Membrane mutants of animal cells: rapid identification of those with a primary defect in glycosylation.

Cloning, sequencing, and functional analysis of oriL, a herpes simplex virus type 1 origin of DNA synthesis.

Nonrandom integration of human U4 RNA pseudogenes.

Ubiquitin is a heat shock protein in chicken embryo fibroblasts.

Stimulation of expression of a herpes simplex virus DNA-binding protein by two viral functions.

Site-specific DNA binding of nuclear factor I: analyses of cellular binding sites.

Suppression of tropomyosin synthesis, a common biochemical feature of oncogenesis by structurally diverse retroviral oncogenes.

Specific regulation of transcription of the discoidin gene family in Dictyostelium discoideum.

TU elements: a heterogeneous family of modularly structured eucaryotic transposons.

Control of types I and II collagen and fibronectin gene expression in chondrocytes delineated by viral transformation.

Monoclonal proliferation of Friend murine leukemia virus-transformed myeloblastic cells occurs early in the leukemogenic process.

Purification and characterization of human H-ras proteins expressed in Escherichia coli.

Characterization, expression, and evolution of the mouse embryonic zeta-globin gene.

Transient gene expression control: effects of transfected DNA stability and trans-activation by viral early proteins.

Differential ability of a T-antigen transport-defective mutant of simian virus 40 to transform primary and established rodent cells.

Surface T-antigen expression in simian virus 40-transformed mouse cells: correlation with cell growth rate.

Low level of cellular protein phosphorylation by nontransforming overproduced p60c-src.

Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells.

Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells.

Nuclei of adenovirus 2-infected cells contain an RNA species that corresponds to an intron excised intact from mRNA precursors.

Processing of the precursor to a chloroplast ribosomal protein made in the cytosol occurs in two steps, one of which depends on a protein made in the chloroplast.

Regulation of protein synthesis factor EF-1 alpha in Mucor racemosus.

Alterations in chromatin structure associated with glucocorticoid-induced expression of endogenous mouse mammary tumor virus genes.

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

Human cellular src gene: nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60c-src.

A 12-O-tetradecanoylphorbol-13-acetate (TPA)-nonproliferative variant of 3T3 cells is resistant to TPA-enhanced gene amplification.

Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.

Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.

Novel chicken actin gene: third cytoplasmic isoform.

Ethidium bromide-induced loss of mitochondrial DNA from primary chicken embryo fibroblasts.

DNA primase-DNA polymerase alpha from simian cells: sequence specificity of initiation sites on simian virus 40 DNA.

Biochemical and immunological characterization of mutant L-M cells with altered levels of dibutyryl cyclic AMP-inducible alkaline phosphatase.

Gene transfer method for transient gene expression, stable transformation, and cotransformation of suspension cell cultures.

The simian virus 40 sequences between 0.169 and 0.423 map units are not essential to immortalize early-passage rat embryo cells.

Functional Analysis of the Transcription Control Region Located Within the Avian Retroviral Long Terminal Repeat

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Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells.

Content Provider	PubMed Central
Author	Johnson, P. J. Coussens, P. M. Shalloway, D. Danko, A. V.
Abstract	NIH 3T3 cells were transfected with plasmids containing Moloney murine leukemia virus long terminal repeats and either chicken c-src or v-src genes. In contrast with the effects observed after transfection with plasmids containing c-src and avian retrovirus or simian virus 40 promoter-enhancers (H. Hanafusa, H. Iba, T. Takeya, and F. R. Cross, p. 1-8, in G. F. Vande Woude, A. J. Levine, W. C. Topp, and J. D. Watson, ed., Cancer Cells, vol. 2, 1984; H. Iba, T. Takeya, F. R. Cross, T. Hanafusa, and H. Hanafusa, Proc. Natl. Acad. Sci. U.S.A. 81:4424-4428, 1984; R. C. Parker, R. Swanstrom, H. E. Varmus, and J. M. Bishop, p. 19-26, in G. F. Vande Woude et al., ed., Cancer Cells, vol. 2, 1984; R. C. Parker, H. E. Varmus, and J. M. Bishop, Cell 37:131-139, 1984; D. Shalloway, P. M. Coussens, and P. Yaciuk, p. 9-17, in G. F. Vande Woude et al., ed., Cancer Cells, vol. 2, 1984; D. Shalloway, P. M. Coussens, and P. Yaciuk, Proc. Natl. Acad. Sci. U.S.A. 81:7071-7075; and K. C. Wilhelmsen, W. G. Tarpley, and H. M. Temin, p. 303-308, in G. F. Vande Woude et al., ed., Cancer Cells, vol. 2, 1984), we found that both types of Moloney murine leukemia virus long terminal repeat-src expression plasmids induced focus formation, although c-src induced only 1% as many foci as v-src. The focus-selected c-src overexpressed cells had altered morphology and limited growth in soft agarose but were not tumorigenic in vivo. Cleveland digests, comparative in vitro kinase assays, secondary transfections, and immunoprecipitations indicated that focus formation was caused by rare transfection events that resulted in very high-level pp60c-src expression rather than by mutations of the transfected c-src genes. These results suggest that pp60v-src induced transformation is not a completely spurious activity which is unrelated to the function of pp60c-src but that it represents a perturbation of already existent molecular control processes involving pp60c-src.
Starting Page	1073
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	5
Volume Number	5
Language	English
Publisher Date	1985-05-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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