NDLI: Bioorthogonal Labeling of 5-Hydroxymethylcytosine in Genomic DNA and Diazirine-Based DNA Photo-Cross-Linking Probes

Content Provider	PubMed Central
Author	Song, Chun-xiao He, Chuan
Abstract	DNA is not merely a combination of four genetic codes, namely A, T, C, and G. It also contains minor modifications that play crucial roles throughout biology. For example, the fifth DNA base, 5-methylcytosine (5-mC), which accounts for ~1% of all the nucleotides in mammalian genomic DNA, is a vital epigenetic mark. It impacts a broad range of biological functions, from development to cancer. Recently, an oxidized form of 5-methylcytosine, 5-hydroxymethylcytosine (5-hmC), was found to constitute the sixth base in the mammalian genome; it was believed to be another crucial epigenetic mark. Unfortunately, further study of this newly discovered DNA base modification has been hampered by inadequate detection and sequencing methods, because current techniques fail to differentiate 5-hmC from 5-mC. The immediate challenge, therefore, is to develop robust methods for ascertaining the positions of 5-hmC within the mammalian genome. In this Account, we describe our development of the first bioorthogonal, selective labeling of 5-hmC to specifically address this challenge.
Related Links	http://dx.doi.org/10.1021/ar2000502
Ending Page	717
Page Count	9
Starting Page	709
File Format	PDF
ISSN	00014842
e-ISSN	15204898
Journal	Accounts of chemical research
Issue Number	9
Volume Number	44
Language	English
Publisher Date	2011-09-20
Access Restriction	Open
Subject Keyword	Chemistry(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine

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Target Identification by Diazirine Photo-Cross-linking and Click Chemistry

Synthesis and evaluation of oligodeoxyribonucleotides containing an aryl(trifluoromethyl)diazirine moiety as the cross-linking probe: photoaffinity labeling of mammalian DNA polymerase beta.

Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA

Methyltransferase-directed derivatization of 5-hydroxymethylcytosine in DNA

5-Hydroxymethylcytosine – the elusive epigenetic mark in mammalian DNA

Synthesis and evaluation of oligodeoxyribonucleotides containing an aryl(trifluoromethyl)diazirine moiety as the cross-linking probe: photoaffinity labeling of mammalian DNA polymerase β

Recent Advances in Target Characterization and Identification by Photoaffinity Probes

5-Hydroxymethylcytosine is a predominantly stable DNA modification

Interfacial Bioorthogonal Cross-Linking

Bioorthogonal Labeling of 5-Hydroxymethylcytosine in Genomic DNA and Diazirine-Based DNA Photo-Cross-Linking Probes

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