NDLI: The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.

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A negative regulatory element with properties similar to those of enhancers is contained within an Alu sequence.

DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae.

Chinese hamster ovary cell mutants with multiple glycosylation defects for production of glycoproteins with minimal carbohydrate heterogeneity.

Context affects nuclear protein localization in Saccharomyces cerevisiae.

Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae.

Functional identification of regulatory elements within the promoter region of platelet-derived growth factor 2.

Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

A cysteine-rich nuclear protein activates yeast metallothionein gene transcription.

Participation of multiple factors, including proliferin, in the inhibition of myogenic differentiation.

Identification of an upstream activating sequence and an upstream repressible sequence of the pyruvate kinase gene of the yeast Saccharomyces cerevisiae.

The replication advantage of a free linear rRNA gene is restored by somatic recombination in Tetrahymena thermophila.

The major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species.

Fidelity of two retroviral reverse transcriptases during DNA-dependent DNA synthesis in vitro.

Control of the Saccharomyces cerevisiae regulatory gene PET494: transcriptional repression by glucose and translational induction by oxygen.

The C proteins of HeLa 40S nuclear ribonucleoprotein particles exist as anisotropic tetramers of (C1)3 C2.

The C-terminal domain of transcription factor IIIA interacts differently with different 5S RNA genes.

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High-resolution mapping of replication fork movement through the amplified dihydrofolate reductase domain in CHO cells by in-gel renaturation analysis.

Replication in the amplified dihydrofolate reductase domain in CHO cells may initiate at two distinct sites, one of which is a repetitive sequence element.

Mapping in vivo topoisomerase I sites on simian virus 40 DNA: asymmetric distribution of sites on replicating molecules.

A system for the analysis of yeast ribosomal DNA mutations.

Differential regulation of rat beta-casein-chloramphenicol acetyltransferase fusion gene expression in transgenic mice.

Cytochrome oxidase subunit V gene of Neurospora crassa: DNA sequences, chromosomal mapping, and evidence that the cya-4 locus specifies the structural gene for subunit V.

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Opposite replication polarity of the germ line c-myc gene in HeLa cells compared with that of two Burkitt lymphoma cell lines.

A ras-dependent pathway abolishes activity of a muscle-specific enhancer upstream from the muscle creatine kinase gene.

Identification of sequences responsible for transcriptional activation of the allantoate permease gene in Saccharomyces cerevisiae.

Purification of a cellular replication factor, RF-C, that is required for coordinated synthesis of leading and lagging strands during simian virus 40 DNA replication in vitro.

Chimeric 3-hydroxy-3-methylglutaryl coenzyme A reductase-dihydrofolate reductase genes display bidirectional expression and unidirectional regulation in stably transfected cells.

Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene.

Definition of the human raf amino-terminal regulatory region by deletion mutagenesis.

Viral DNA synthesis in nonpermissive rat F-111 cells and its role in neoplastic transformation by polyomavirus.

The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.

Inhibition of tyrosine kinase activity of the epidermal growth factor (EGF) receptor by a truncated receptor form that binds to EGF: role for interreceptor interaction in kinase regulation.

Characterization of mutant mitochondrial plasmids of Neurospora spp. that have incorporated tRNAs by reverse transcription.

Levels of RNA from a family of putative serine protease genes are reduced in Drosophila melanogaster dunce mutants and are regulated by cyclic AMP.

The bcl-2 candidate proto-oncogene product is a 24-kilodalton integral-membrane protein highly expressed in lymphoid cell lines and lymphomas carrying the t(14;18) translocation.

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Molecular isolation and analysis of the erect wing locus in Drosophila melanogaster.

The regulated production of mu m and mu s mRNA is dependent on the relative efficiencies of mu s poly(A) site usage and the c mu 4-to-M1 splice.

Negative control region at the 5' end of murine leukemia virus long terminal repeats.

Octamer transcription factors 1 and 2 each bind to two different functional elements in the immunoglobulin heavy-chain promoter.

IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae.

Posttranscriptional stabilization of c-fms mRNA by a labile protein during human monocytic differentiation.

Proteins binding to site C2 (muE3) in the immunoglobulin heavy-chain enhancer exist in multiple oligomeric forms.

Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos.

Retrovirus-mediated transfer and expression of the interleukin-3 gene in mouse hematopoietic cells result in a myeloproliferative disorder.

A yeast mutation that stabilizes a plasmid bearing a mutated ARS1 element.

Dual functions of a cis-acting element within the rat prolactin gene promoter.

A yeast protein possesses the DNA-binding properties of the adenovirus major late transcription factor.

Individual protein-binding domains of the insulin gene enhancer positively activate beta-cell-specific transcription.

Methylation of replicating and nonreplicating DNA in the ciliate Tetrahymena thermophila.

A protein kinase C cDNA without the regulatory domain is active after transfection in vivo in the absence of phorbol ester.

Structural organization of upstream exons and distribution of transcription start sites in the chicken c-myb gene.

NF-kappa B binds within a region required for B-cell-specific expression of major histocompatibility complex class II gene E alpha d.

Enhancement of major histocompatibility class I protein synthesis by DNA damage in cultured human fibroblasts and keratinocytes.

Induction of beta-polymerase mRNA by DNA-damaging agents in Chinese hamster ovary cells.

Transgenic mice carrying the mouse mammary tumor virus ras fusion gene: distinct effects in various tissues.

Transforming growth factor alpha: an aromatic side chain at position 38 is essential for biological activity.

Detection of a Myc-associated protein by chemical cross-linking.

Cell cycle regulation of H2b histone octamer DNA-binding activity in Chinese hamster lung fibroblasts.

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The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.

Content Provider	PubMed Central
Author	Bernstein, P. Peltz, S. W. Ross, J.
Abstract	Using an in vitro mRNA decay system, we investigated how poly(A) and its associated poly(A)-binding protein (PABP) affect mRNA stability. Cell extracts used in the decay reactions were depleted of functional PABP either by adding excess poly(A) competitor or by passing the extracts over a poly(A)-Sepharose column. Polyadenylated mRNAs for beta-globin, chloramphenicol acetyltransferase, and simian virus 40 virion proteins were degraded 3 to 10 times faster in reactions lacking PABP than in those containing excess PABP. The addition of purified Saccharomyces cerevisiae or human cytoplasmic PABP to PABP-depleted reactions stabilized the polyadenylated mRNAs. In contrast, the decay rates of nonpolyadenylated mRNAs were unaffected by PABP, indicating that both the poly(A) and its binding protein were required for maintaining mRNA stability. A nonspecific single-stranded binding protein from Escherichia coli did not restore stability to polyadenylated mRNA, and the stabilizing effect of PABP was inhibited by anti-PABP antibody. The poly(A) tract was the first mRNA segment to be degraded in PABP-depleted reactions, confirming that the poly(A)-PABP complex was protecting the 3' region from nucleolytic attack. These results indicate that an important function of poly(A), in conjunction with its binding protein, is to protect polyadenylated mRNAs from indiscriminate destruction by cellular nucleases. A model is proposed to explain how the stability of an mRNA could be affected by the stability of its poly(A)-PABP complex.
Starting Page	659
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	2
Volume Number	9
Language	English
Publisher Date	1989-02-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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