NDLI: Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae.

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Structure and expression of STK, a src-related gene in the simple metazoan Hydra attenuata.

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Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA.

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Mammalian genes coordinately regulated by growth arrest signals and DNA-damaging agents.

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Identification of an octamer-binding site in the mouse kappa light-chain immunoglobulin enhancer.

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Novel regulation of transcription initiation of the peptide IX gene of adenovirus 2.

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Mapping of branch sites in trans-spliced pre-mRNAs of Trypanosoma brucei.

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Possible involvement of normal p21 H-ras in the insulin/insulinlike growth factor 1 signal transduction pathway.

Transactivation of gene expression by nuclear and cytoplasmic rel proteins.

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A small nuclear RNA, U5, can transform cells in vitro.

Expression of a human multidrug resistance cDNA (MDR1) in the bone marrow of transgenic mice: resistance to daunomycin-induced leukopenia.

cis-acting elements and a trans-acting factor affecting alternative splicing of adenovirus L1 transcripts.

Nuclear localization of the adenovirus DNA-binding protein: requirement for two signals and complementation during viral infection.

Translational control of germ cell-expressed mRNA imposed by alternative splicing: opioid peptide gene expression in rat testis.

Distinguishable promoter elements are involved in transcriptional activation by E1a and cyclic AMP.

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An intronic 10-base-pair deletion in a class II A beta gene affects RNA processing.

Analysis by cell-free transcription of the liver-specific pyruvate kinase gene promoter.

A natural case of RIP: degeneration of the DNA sequence in an ancestral tandem duplication.

Accurate processing of human pre-rRNA in vitro.

Heteroduplex DNA correction in Saccharomyces cerevisiae is mismatch specific and requires functional PMS genes.

Alterations in tyrosine protein phosphorylation induced by antibody-mediated cross-linking of the CD4 receptor of T lymphocytes.

Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae.

Novel N-terminal amino acid sequence required for retention of a hepatitis B virus glycoprotein in the endoplasmic reticulum.

Translation in Saccharomyces cerevisiae: initiation factor 4E-dependent cell-free system.

Mutations of the platelet-derived growth factor receptor that cause a loss of ligand-induced conformational change, subtle changes in kinase activity, and impaired ability to stimulate DNA synthesis.

Ribozyme, antisense RNA, and antisense DNA inhibition of U7 small nuclear ribonucleoprotein-mediated histone pre-mRNA processing in vitro.

Time of replication of ARS elements along yeast chromosome III.

E2F from adenovirus-infected cells binds cooperatively to DNA containing two properly oriented and spaced recognition sites.

Regulation of alpha-factor production in Saccharomyces cerevisiae: a-factor pheromone-induced expression of the MF alpha 1 and STE13 genes.

Insertional activation of N-myc by endogenous Moloney-like murine retrovirus sequences in macrophage cell lines derived from myeloma cell line-macrophage hybrids.

Mating-type control in Saccharomyces cerevisiae: isolation and characterization of mutants defective in repression by a1-alpha 2.

Insulin gene enhancer activity is inhibited by adenovirus 5 E1a gene products.

Identification of a yeast protein with properties similar to those of the immunoglobulin heavy-chain enhancer-binding protein NF-muE3.

Mock retroviral infection alters the developmental potential of murine bone marrow stem cells.

Expression of the yes proto-oncogene in cerebellar Purkinje cells.

Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.

Subcellular localization of an intermediate filament protein and its mRNA in glial cells.

Identification of a germ line transcript from the unrearranged kappa gene in human B cells.

Developmentally regulated use of alternative promoters creates a novel platelet-derived growth factor receptor transcript in mouse teratocarcinoma and embryonic stem cells.

Identification of a new promoter upstream of the murine dihydrofolate reductase gene.

Elongation factor EF-1 alpha gene dosage alters translational fidelity in Saccharomyces cerevisiae.

Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens.

Beta-globin enhancers target expression of a heterologous gene to erythroid tissues of transgenic mice.

The U1 RNA-Binding Site of the U1 Small Nuclear Ribonucleoprotein (snRNP)-Associated A Protein Suggests a Similarity with U2 snRNPs

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Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae.

Content Provider	PubMed Central
Author	Kunkel, T. A. Hamatake, R. K. Motto-fox, J. Fitzgerald, M. P. Sugino, A.
Abstract	We have determined the fidelity of DNA synthesis by DNA polymerase I (yPol I) from Saccharomyces cerevisiae. To determine whether subunits other than the polymerase catalytic subunit influence fidelity, we measured the accuracy of yPol I purified by conventional procedures, which yields DNA polymerase with a partially proteolyzed catalytic subunit and no associated primase activity, and that of yPol I purified by immunoaffinity chromatography, which yields polymerase having a single high-molecular-weight species of the catalytic subunit, as well as three additional polypeptides and DNA primase activity. In assays that score polymerase errors within the lacZ alpha-complementation gene in M13mp2 DNA, yPol I and the yPol I-primase complex produced single-base substitutions, single-base frameshifts, and larger deletions. For specific errors and template positions, the two forms of polymerase exhibited differences in fidelity that could be as large as 10-fold. Nevertheless, results for the overall error frequency and the spectrum of errors suggest that the yPol I-DNA primase complex is not highly accurate and that, just as for the polymerase alone, its fidelity is not sufficient to account for a low spontaneous mutation rate in vivo. The specificity data also suggest models to explain -1 base frameshifts in nonrepeated sequences and certain complex deletions by a direct repeat mechanism involving aberrant loop-back synthesis.
Starting Page	4447
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	10
Volume Number	9
Language	English
Publisher Date	1989-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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