NDLI: Biomimetic Polymer Brushes Containing Tethered Acetylcholine Analogs for Protein and Hippocampal Neuronal Cell Patterning

Content Provider	PubMed Central
Author	Zhou, Zhaoli Yu, Panpan Geller, Herbert M. Ober, Christopher K.
Abstract	This paper describes a method to control neuronal cell adhesion and differentiation with both chemical and topographic cues by using a spatially defined polymer brush pattern. First, biomimetic methacrylate polymer brushes containing tethered neurotransmitter acetylcholine functionalities in the form of dimethylaminoethyl methacrylate (DMAEMA), or free hydroxyl-terminated poly(ethylene glycol) (PEG) units were prepared using the “grown from” method through surface-initiated atom transfer radical polymerization (SI-ATRP) reactions. The surface properties of the resulting brushes were thoroughly characterized with various techniques and hippocampal neuronal cell culture on the brush surfaces exhibit cell viability and differentiation comparable to, or even better than, those on commonly used poly-L-lysine coated glass coverslips. The polymer brushes were then patterned via UV photolithography techniques to provide specially designed surface features with different sizes (varying from 2 µm to 200 µm) and orientations (horizontal and vertical). Protein absorption experiments and hippocampal neuronal cell culture tests on the brush patterns showed that both protein and neurons can adhere to the patterns and therefore be guided by such patterns. These results also demonstrate that, because of their unique chemical composition and well-defined nature, the developed polymer brushes may find many potential applications in cell-material interactions studies and neural tissue engineering.
Related Links	http://dx.doi.org/10.1021/bm301785b
Ending Page	537
Page Count	9
Starting Page	529
File Format	PDF
ISSN	15257797
e-ISSN	15264602
Journal	Biomacromolecules
Issue Number	2
Volume Number	14
Language	English
Publisher Date	2013-02-11
Access Restriction	Open
Subject Keyword	Materials Chemistry Bioengineering Polymers and Plastics Biomaterials Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Materials Chemistry Biomaterials Bioengineering Polymers and Plastics

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